Isotype controls are a type of primary antibodies that lack specificity to the target, but match the class and type of the primary antibody used in in vitro and in vivo studies. Isotype controls are often used to measure the level of non-specific background signal caused by binding non-specifically to Fc receptors on the cell surface, or other cellular molecules.
Chinese hamster ovary (CHO) host cell line is the most popular mammalian system of therapeutic proteins production. The isotype controls produced by CHO line share similar posttranslational modifications with the therapeutic proteins, especially in N‐linked glycosylation, which are useful benchmark in the discovery and development of therapeutic antibodies.
