| Catalog Number | KC-0152 |
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| Cell Line Name | U2OS-REV-GFP-Cell-Line |
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| Host Cell Line | Human U2OS cell line |
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| Description | Stable U2OS clone expressing exogenous human GFP tagged REV |
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| Quantity | One vial of frozen cells (≥2-106/vial) |
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| Stability | Stable in culture over a minimum of 10 passages |
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| Application | Drug screening and biological assays |
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| Freezing Medium | 70% McCoy's 5a+20%FBS+10%DMSO |
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| Propagation Medium | McCoy's 5a+10%FBS+2µg/mL Puromycin |
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| Selection Marker | Puromycin |
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| Subculture | Split saturated culture 1:3-1:6 every 3 days; seed out at about 1-3 × 105 cells/mL |
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| Incubation | 37 °C with 5% CO2 |
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| Storage | Liquid nitrogen immediately upon receiving |
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| Mycoplasma Status | Negative |
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| In Vivo Validation | NA |
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