KC-0380

MCF7-Cell-Line-(Not-for-sale)

20313

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Background of MCF7-Cell-Line-(Not-for-sale)

MCF7 was established from the pleural effusion of a 69-year-old woman with metastatic invasive breast adenocarcinoma (after radio- and hormone therapy) in 1970; widely used and extensively characterized cell line; described as ER-positive, but HER2neu negative; listed in the NCI cancer cell line panel and in the CCLE (ref 18232); a derivative expressing GFP is available (ACC 983); RNA sequencing data are available (see Ref 40906 and RNA-Seq)

Specifications

Catalog Number	KC-0380
Cell Line Name	MCF7-Cell-Line-(Not-for-sale)
Host Cell Line	NA
Description	Human breast adenocarcinoma cell line, established from the pleural effusion of a 69-year-old woman with metastatic mammary carcinoma
Quantity	One vial of frozen cells (≥2-10⁶/vial)
Stability	Stable in culture over a minimum of 10 passages
Application	3D cell culture; Cancer research
Freezing Medium	70% EMEM+20% FBS+10% DMSO
Propagation Medium	EMEM+10%FBS+0.01 mg/ml insulin
Selection Marker	NA
Morphology	Epithelial
Subculture	Split saturated culture 1:2-1:4 every 3-4 days; seed out at about 1-3 × 10⁵ cells/mL
Incubation	37 °C with 5% CO₂
Storage	Liquid nitrogen immediately upon receiving
Doubling Time	Approximately 50 hours
Mycoplasma Status	Negative
In Vivo Validation	Yes

Cell Line Generation

Characterization

Cell Resuscitation

Prewarm culture medium (EMEM+10%FBS+0.01 mg/ml insulin)in a 37°C water bath.
Thaw the frozen vial in a 37°C water bath for 1-2 minutes.
Transfer the vial into biosafety cabinet, and wipe the surface with 70% ethanol.
Unscrew the top of the vial and transfer the cell suspension gently into a sterile centrifuge tube containing 9.0mL complete culture medium.
Spin at ~ 125 × g for 5-7 minutes at room temperature, and discard the supernatant without disturbing the pellet.
Resuspend cell pellet with the appropriate volume of complete medium and transfer the cell suspension into a T25 culture flask.
Incubate the flask at 37°C, 5% CO₂ incubator.
Split saturated culture 1:2-1:4 every 3-4 days; seed out at about 1-3 × 10⁵ cells/mL.

Cell Freezing

Prepare the freezing medium (70% EMEM + 20% FBS + 10% DMSO) fresh immediately before use.
Keep the freezing medium on ice and label cryovials.
Transfer cells to a sterile, conical centrifuge tube, and count the cells.
Centrifuge the cells at 250×g for 5 minutes at room temperature and carefully aspirate off the medium.
Resuspend the cells at a density of at least 3×10⁶ cells/mL in chilled freezing medium.
Aliquot 1 mL of the cell suspension into each cryovial.
Freeze cells in the CoolCell freezing container overnight in a -80°C freezer.
Transfer vials to liquid nitrogen for long-term storage

References

Parallel genome-scale loss of function screens in 216 cancer cell lines for the identification of context-specific genetic dependencies.Sci. Data 1:140035-140035(2014)
The effect of weightlessness on cytoskeleton architecture and proliferation of human breast cancer cell line MCF-7.FASEB J. 15:1104-1106(2001)

Use License Agreement

Research Use Only.

Not for use in diagnostic procedures or therapeutic applications.

Redistribution of the cell line or its derivatives is prohibited without prior written permission from Kyinno Biotechnology.