| Catalog Number | KC-1372 |
|---|
| Cell Line Name | 293T-cyno-SIGLEC15-Cell-Line |
|---|
| Host Cell Line | Human HEK293T cell line |
|---|
| Description | Stable HEK293T clone expressing exogenous crab-eating macaque SIGLEC15 |
|---|
| Quantity | Two vials of frozen cells (≥2-106/vial) |
|---|
| Stability | Stable in culture over a minimum of 10 passages |
|---|
| Application | Drug screening and biological assays |
|---|
| Freezing Medium | 70% DMEM+20% FBS+10% DMSO |
|---|
| Propagation Medium | DMEM+10%FBS+1µg/mL Puromycin |
|---|
| Selection Marker | Puromycin |
|---|
| Morphology | Epithelial |
|---|
| Subculture | Split saturated culture 1:3-1:6 every 3 days; seed out at about 1-3 × 105 cells/mL |
|---|
| Incubation | 37 °C with 5% CO2 |
|---|
| Storage | Liquid nitrogen immediately upon receiving |
|---|
| Doubling Time | Approximately 30 hours |
|---|
| Mycoplasma Status | Negative |
|---|
| In Vivo Validation | NA |
|---|
