KC-1547

Jurkat-NFAT-Luc2-BTLA-CD3-zeta-Cell-Line

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Home » 细胞系 » Jurkat-NFAT-Luc2-BTLA-CD3-zeta-Cell-Line

Background of Jurkat-NFAT-Luc2-BTLA-CD3-zeta-Cell-Line

Specifications

Catalog NumberKC-1547
Cell Line NameJurkat-NFAT-Luc2-BTLA-CD3-zeta-Cell-Line
Host Cell LineHuman Jurkat cell line
DescriptionStable Jurkat clone expressing exogenous human BTLA and CD3 zeta as well as luciferase under the control of NFAT signaling pathway
QuantityTwo vials of frozen cells (≥2-106/vial)
StabilityStable in culture over a minimum of 10 passages
ApplicationDrug screening and biological assays
Freezing Medium70% RPMI1640+20% FBS+10% DMSO
Propagation MediumRPMI1640+10%FBS+300µg/mL Hygromycin B+0.75µg/mL Puromycin
Selection MarkerPuromycin
Incubation37 °C with 5% CO2
StorageLiquid nitrogen immediately upon receiving
Mycoplasma StatusNegative
In Vivo ValidationNA

Cell Line Generation

Characterization

Cell Resuscitation

Cell Freezing

References

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