KC-1793

CHOK1-5HTR1F-CRE-Luc2-Reporter-Cell-Line

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Home » 细胞系 » CHOK1-5HTR1F-CRE-Luc2-Reporter-Cell-Line

Background of CHOK1-5HTR1F-CRE-Luc2-Reporter-Cell-Line

Specifications

Catalog NumberKC-1793
Cell Line NameCHOK1-5HTR1F-CRE-Luc2-Reporter-Cell-Line
Host Cell LineChinese hamster ovary CHO-K1 cell line
DescriptionStable CHO-K1 clone expressing exogenous human 5HTR1F and luciferase under the control of CRE response element
QuantityTwo vials of frozen cells (≥2-106/vial)
StabilityStable in culture over a minimum of 10 passages
ApplicationDrug screening and biological assays
Freezing Medium70% RPMI1640+20% FBS+10% DMSO
Propagation MediumRPMI1640+10%FBS+10µg/mL Puromycin+750µg/mL Hygromycin B
Selection MarkerPuromycin
MorphologyEpithelial-like
SubcultureSplit saturated culture 1:4-1:8 every 2-3 days; seed out at about 1-2 × 105 cells/mL
Incubation37 °C with 5% CO2
StorageLiquid nitrogen immediately upon receiving
Mycoplasma StatusNegative
In Vivo ValidationNA

Cell Line Generation

Characterization

Cell Resuscitation

Cell Freezing

References

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