KC-2037

HEK293T-EWI2-KO-1A1-Cell-Line

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Home » 细胞系 » HEK293T-EWI2-KO-1A1-Cell-Line

Background of HEK293T-EWI2-KO-1A1-Cell-Line

Specifications

Catalog NumberKC-2037
Cell Line NameHEK293T-EWI2-KO-1A1-Cell-Line
Host Cell LineHuman HEK293T cell line
DescriptionStable HEK293T clone with human EW12 gene knockout, No.1A1
QuantityTwo vials of frozen cells (≥2-106/vial)
StabilityStable in culture over a minimum of 10 passages
ApplicationDrug screening and biological assays
Freezing Medium70% DMEM+20% FBS+10% DMSO
Propagation MediumDMEM+10%FBS
MorphologyEpithelial
SubcultureSplit saturated culture 1:3-1:6 every 3 days; seed out at about 1-3 × 105 cells/mL
Incubation37 °C with 5% CO2
StorageLiquid nitrogen immediately upon receiving
Doubling TimeApproximately 30 hours
Mycoplasma StatusNegative
In Vivo ValidationNA

Cell Line Generation

Characterization

Cell Resuscitation

Cell Freezing

References

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