KC-2224

CHO-K1-cyno-TREM2-DAP12-Cell-Line

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Background of CHO-K1-cyno-TREM2-DAP12-Cell-Line

TERM2, full named as Triggering receptor expressed on myeloid cells 2, is a membrane protein which expressed primarily in immune cells across many different tissues, and form a receptor signaling complex with TYROBP, also named as DAP12, then mediates signaling and cell activation after binding serval ligands, it plays an important role in different inflammatory diseases, especially Alzheimer's disease, and is also believed as a promising target for cancer therapy.

Specifications

Catalog Number	KC-2224
Cell Line Name	CHO-K1-cyno-TREM2-DAP12-Cell-Line
Host Cell Line	Chinese hamster ovary CHO-K1 cell line
Description	Stable CHOK1 cell line expressing exogenous cyno TREM2-DAP12 gene
Quantity	Two vials of frozen cells (≥2-10⁶/vial)
Stability	Stable in culture over a minimum of 10 passages
Application	Drug screening and biological assays
Freezing Medium	70% RPMI1640+20% FBS+10% DMSO
Propagation Medium	RPMI1640+10%FBS+10µg/mL Puromycin
Selection Marker	Puromycin
Morphology	Epithelial-like
Subculture	Split saturated culture 1:4-1:8 every 2-3 days; seed out at about 1-2 × 10⁵ cells/mL
Incubation	37 °C with 5% CO₂
Storage	Liquid nitrogen immediately upon receiving
Doubling Time	Approximately 24 hours
Mycoplasma Status	Negative
In Vivo Validation	NA

Cell Line Generation

CHOK1 cyno TREM2-DAP12 cell line was generated using a lentiviral vector expressing the cyno TREM2-DAP12 sequence.

Characterization

Figure: Characterization of TREM2 overexpression in CHOK1 stable clones using FACS.

Cell Resuscitation

Prewarm culture medium (RPMI1640 + 10% FBS + 10µg/mL Puromycin) in a 37°C water bath.
Thaw the frozen vial in a 37°C water bath for 1-2 minutes.
Transfer the vial into biosafety cabinet, and wipe the surface with 70% ethanol.
Unscrew the top of the vial and transfer the cell suspension gently into a sterile centrifuge tube containing 9.0mL complete culture medium.
Spin at ~ 125 × g for 5-7 minutes at room temperature, and discard the supernatant without disturbing the pellet.
Resuspend cell pellet with the appropriate volume of complete medium and transfer the cell suspension into a T25 culture flask.
Incubate the flask at 37°C, 5% CO₂ incubator.
Split saturated culture 1:4-1:8 every 2-3 days; seed out at about 1-2 × 10⁵ cells/mL.

Cell Freezing

Prepare the freezing medium (70% RPMI-1640 + 20% FBS + 10% DMSO) fresh immediately before use.
Keep the freezing medium on ice and label cryovials.
Transfer cells to a sterile, conical centrifuge tube, and count the cells.
Centrifuge the cells at 250×g for 5 minutes at room temperature and carefully aspirate off the medium.
Resuspend the cells at a density of at least 3×10⁶ cells/mL in chilled freezing medium.
Aliquot 1 mL of the cell suspension into each cryovial.
Freeze cells in the CoolCell freezing container overnight in a -80°C freezer.
Transfer vials to liquid nitrogen for long-term storage

References

Deczkowska, A., Weiner, A. & Amit, I. The Physiology, Pathology, and Potential Therapeutic Applications of the TREM2 Signaling Pathway. Cell 181, 1207Ã¿1217 (2020).
Yang, J. et al. TREM2 ectodomain and its soluble form in Alzheimer£ªs disease. J Neuroinflammation 17, 204 (2020).