| Catalog Number | KC-2425 |
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| Cell Line Name | 293T-mouse-4Ig-B7H3-Middle |
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| Host Cell Line | Human HEK293T cell line |
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| Quantity | Two vials of frozen cells (≥2-106/vial) |
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| Stability | Stable in culture over a minimum of 10 passages |
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| Application | Drug screening and biological assays |
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| Freezing Medium | 70% DMEM+20% FBS+10% DMSO |
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| Propagation Medium | DMEM+10%FBS+1μg/mL Puromycin |
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| Selection Marker | Puromycin |
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| Morphology | Epithelial |
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| Subculture | Split saturated culture 1:3-1:6 every 3 days; seed out at about 1-3 × 105 cells/mL |
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| Incubation | 37 °C with 5% CO2 |
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| Storage | Liquid nitrogen immediately upon receiving |
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| Doubling Time | Approximately 30 hours |
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| Mycoplasma Status | Negative |
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| In Vivo Validation | NA |
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