| Catalog Number | KC-3033 |
|---|---|
| Cell Line Name | Ba/F3-ETV6-BTK-C481S-Luc2-Cell-Line |
| Host Cell Line | Mouse Ba/F3 cell line |
| Description | Stable Ba/F3 clone expressing exogenous ETV6-BTK fusion gene with C481S mutation and luciferase. |
| Quantity | Two vials of frozen cells (≥2-106/vial) |
| Stability | Stable in culture over a minimum of 10 passages |
| Application | Drug screening and biological assays |
| Freezing Medium | 70% RPMI1640+20% FBS+10% DMSO |
| Propagation Medium | RPMI1640+10%FBS+1000µg/mL Hygromycin B |
| Selection Marker | Hygromycin B |
| Morphology | Mostly single, round (some polymorph) cells in suspension |
| Subculture | Split saturated culture 1:10 every 3 days; seed out at about 1-3 × 105 cells/mL |
| Incubation | 37 °C with 5% CO2 |
| Storage | Liquid nitrogen immediately upon receiving |
| Doubling Time | Approximately 20 hours |
| Mycoplasma Status | Negative |
| In Vivo Validation | NA |
