KB-1397

SIM0235-hIgG1

33709

Background of SIM0235-hIgG1

SIM0235 represents a humanized immunoglobulin G1 (IgG1) monoclonal antibody with a specific affinity toward and inhibition of TNFR2. This antibody showcases the ability to effectively eliminate TNFR2-expressing immunosuppressive cells, encompassing regulatory T cells (Tregs) and bone marrow-derived suppressor cells. Its mechanism involves the utilization of antibody-dependent cellular cytotoxicity (ADCC), antibody-dependent cellular phagocytosis (ADCP), and other Fc-receptor functions. Moreover, this remarkable molecule demonstrates the capability to impede TNFR2 activation by endogenous tumor necrosis factor (TNF), thereby exerting influence over the immunosuppressive function and proliferation typically mediated by TNFR2. Consequently, this augmentation serves to bolster the body's immune response against tumors. Notably, SIM0235 exhibits an additional proficiency in binding to TNFR2 present on the surface of tumor cells, subsequently effecting their direct elimination.

Specifications

Catalog Number	KB-1397
Antibody Name	SIM0235-hIgG1
Isotype	Human IgG1,kappa
Target	TNFR2
Species Reactivity	Human
Host Cell Line	EXPI CHO
Purification Method	Affinity purified
Concentration	>2 mg/mL
Formulation	50 mM sodium citrate,150mM NaCl,pH5.5
Purity	>95% by SDS-PAGE and SEC-HPLC
Validation	ELISA
Endotoxin Level	<0.2 EU/mg as determined by the LAL method
Sterility	0.2μm filtered
Storage	It is recommended that the protein should be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Stability	Stable for twelve months from date of receipt when stored at -20°C to -80°C; Stored at 2-8°C for one month without detectable loss of activity.

Characterization

Application

References

Rauert H, Wicovsky A, Muller N, Siegmund D, Spindler V, Waschke J, et al.. Membrane Tumor Necrosis Factor (TNF) Induces P100 Processing via TNF Receptor-2 (TNFR2). J Biol Chem (2010) 285:7394–404.
Shamdani S, Uzan G, Naserian S. TNFalpha-TNFR2 Signaling Pathway in Control of the Neural Stem/Progenitor Cell Immunosuppressive Effect: Different Experimental Approaches to Assess This Hypothetical Mechanism Behind Their Immunological Function. Stem Cell Res Ther (2020) 11:307.