KC-2869

293T-CDH17-Cell-Line

Stable HEK293T cell line expressing exogenous human CDH17 gene

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Background of 293T-CDH17-Cell-Line

CDH17, also known as HPT1, is a protein that belongs to the cadherin family of calcium-dependent cell adhesion molecules. CDH17 is a component of the gastrointestinal tract and pancreatic ducts, acting as an intestinal protondependent peptide transporter in the first step in oral absorption of many medically important peptide-based drugs. CDH17 may also play a role in the morphological organization of liver and intestine.

Specifications

Catalog Number	KC-2869
Cell Line Name	293T-CDH17-Cell-Line
NCBI/UniProt Accession Number	NM_001144663.2
Clone Number	5#
Host Cell Line	293T
Description	Stable HEK293T cell line expressing exogenous human CDH17 gene
Quantity	Two vials of frozen cells (≥2-10⁶/vial)
Stability	Stable in culture over a minimum of 10 passages
Application	Drug screening and biological assays
Freezing Medium	70% DMEM + 20% FBS + 10% DMSO
Propagation Medium	DMEM + 10% FBS + 1μg/mL Puromycin
Selection Marker	Puromycin
Morphology	Epithelial
Subculture	Split saturated culture 1:4-1:5 every 2-3 days; seed out at about 1-3 × 10⁵ cells/mL
Incubation	37 °C with 5% CO₂
Storage	Liquid nitrogen immediately upon receiving
Doubling Time	Approximately 30 hours
Mycoplasma Status	Negative
In Vivo Validation	NA

Cell Line Generation

293T human CDH17 Cell Line was generated using a lentiviral vector expressing the human CDH17 sequence.

Characterization

Figure: Characterization of human CDH17 overexpression in 293T stable clones using FACS.

Cell Resuscitation

Prewarm culture medium (DMEM supplemented with 10% FBS and 1μg/mL Puromycin)in a 37°C water bath.
Thaw the frozen vial in a 37°C water bath for 1-2 minutes.
Transfer the vial into biosafety cabinet, and wipe the surface with 70% ethanol.
Unscrew the top of the vial and transfer the cell suspension gently into a sterile centrifuge tube containing 9.0mL complete culture medium.
Spin at ~ 125 × g for 5-7 minutes at room temperature, and discard the supernatant without disturbing the pellet.
Resuspend cell pellet with the appropriate volume of complete medium and transfer the cell suspension into a T25 culture flask.
Incubate the flask at 37°C, 5% CO₂ incubator.
Split saturated culture 1:4-1:5 every 2-3 days; seed out at about 1-3 × 10⁵ cells/mL.

Cell Freezing

Prepare the freezing medium (70% DMEM + 20% FBS + 10% DMSO) fresh immediately before use.
Keep the freezing medium on ice and label cryovials.
Transfer cells to a sterile, conical centrifuge tube, and count the cells.
Centrifuge the cells at 250×g for 5 minutes at room temperature and carefully aspirate off the medium.
Resuspend the cells at a density of at least 3×10⁶ cells/mL in chilled freezing medium.
Aliquot 1 mL of the cell suspension into each cryovial.
Freeze cells in the CoolCell freezing container overnight in a -80°C freezer.
Transfer vials to liquid nitrogen for long-term storage

References

Entrez Gene: CDH17 cadherin 17, LI cadherin (liver-intestine).
Kremmidiotis G, Baker E, Crawford J, Eyre HJ, Nahmias J, Callen DF (Aug 1998). Localization of human cadherin
genes to chromosome regions exhibiting cancer-related loss of heterozygosity. Genomics. 49 (3): 467–71.
doi:10.1006/geno.1998.5281. PMID 9615235.
Chalmers IJ, Hofler H, Atkinson MJ (Jun 1999). Mapping of a cadherin gene cluster to a region of chromosome
5 subject to frequent allelic loss in carcinoma. Genomics. 57 (1): 160–3. doi:10.1006/geno.1998.5717. PMID