KC-3231

PanO2-CDH17 Cell Line

34236

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Background of PanO2-CDH17 Cell Line

CDH17 is a member of the cadherin superfamily, genes encoding calcium-dependent, membrane-associated glycoproteins. The protein is a component of the gastrointestinal tract and pancreatic ducts, is a highly specific marker for gastrointestinal adenocarcinoma and may be important in clinical diagnosis. The protein may also play a role in the morphological organization of liver and intestine.

Specifications

Catalog Number	KC-3231
Cell Line Name	PanO2-CDH17 Cell Line
Host Cell Line	PanO2
Description	Stable PanO2 clone expressing exogenous CDH17 gene
Quantity	One vial of frozen cells (≥2-10⁶/vial)
Stability	Stable in culture over a minimum of 10 passages
Application	Drug screening and biological assays
Freezing Medium	70% RPMI1640 + 20% FBS + 10% DMSO
Propagation Medium	RPMI1640 + 10% FBS + 2ug/ml Puromycin
Selection Marker	Puromycin
Morphology	Fibroblastoid cells growing as a monolayer
Subculture	Split saturated culture 1:4-1:5 every 2-3 days; seed out at about 1-3 × 10⁵ cells/mL
Incubation	37 °C with 5% CO₂
Storage	Liquid nitrogen immediately upon receiving
Doubling Time	Approximately 30 hours
Mycoplasma Status	Negative
In Vivo Validation	NA

Cell Line Generation

PanO2 CDH17 cell line was generated using a lentiviral vector expressing the CDH17 sequence.

Characterization

Figure 1: Characterization of endogenous CDH17 expression in PanO2 cells using FACS.

Figure 2: Characterization of CDH17 overexpression in the PanO2 CDH17 stable clone using FACS.

Cell Resuscitation

1. Prewarm culture medium (RPMI1640 supplemented with 10% FBS and 2μg/mL puromycin)in a 37°C water bath. 2. Thaw the frozen vial in a 37°C water bath for 1-2 minutes. 3. Transfer the vial into biosafety cabinet, and wipe the surface with 70% ethanol. 4. Unscrew the top of the vial and transfer the cell suspension gently into a sterile centrifuge tube containing 9.0mL complete culture medium. 5. Spin at ~ 125 × g for 5-7 minutes at room temperature, and discard the supernatant without disturbing the pellet. 6. Resuspend cell pellet with the appropriate volume of complete medium and transfer the cell suspension into a T25 culture flask. 7. Incubate the flask at 37°C, 5% CO₂ incubator. 8. Split saturated culture 1:4-1:5 every 2-3 days; seed out at about 1-3 × 10⁵ cells/mL.

Cell Freezing

1. Prepare the freezing medium (70% RPMI1640 + 20% FBS + 10% DMSO) fresh immediately before use. 2. Keep the freezing medium on ice and label cryovials. 3. Transfer cells to a sterile, conical centrifuge tube, and count the cells. 4. Centrifuge the cells at 250×g for 5 minutes at room temperature and carefully aspirate off the medium. 5. Resuspend the cells at a density of at least 3×10⁶ cells/mL in chilled freezing medium. 6. Aliquot 1 mL of the cell suspension into each cryovial. 7. Freeze cells in the CoolCell freezing container overnight in a -80°C freezer. 8. Transfer vials to liquid nitrogen for long-term storage.

References

1.Yakirevich E, Magi-Galluzzi C, Grada Z, Lu S, Resnick MB, Mangray S. Cadherin 17 is a sensitive and specific marker for metanephric adenoma. Am J Surg Pathol. 2015 Apr;39(4):479-86. doi: 10.1097/PAS.0000000000000401. PMID: 25768256; PMCID: PMC4360917. 2. Bartolomé RA, Barderas R, Torres S, Fernandez-Aceñero MJ, Mendes M, García-Foncillas J, Lopez-Lucendo M, Casal JI. Cadherin-17 interacts with α2β1 integrin to regulate cell proliferation and adhesion in colorectal cancer cells causing liver metastasis. Oncogene. 2014 Mar 27;33(13):1658-69. doi: 10.1038/onc.2013.117. Epub 2013 Apr 22. PMID: 23604127.

Use License Agreement

Research Use Only.

Not for use in diagnostic procedures or therapeutic applications.

Redistribution of the cell line or its derivatives is prohibited without prior written permission from Kyinno Biotechnology.