KC-3688

293T-CDH2 Cell Line

34616

Home » 293T-CDH2 Cell Line

Background of 293T-CDH2 Cell Line

This gene encodes a classical cadherin and member of the cadherin superfamily. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein is proteolytically processed to generate a calcium-dependent cell adhesion molecule and glycoprotein. This protein plays a role in the establishment of left-right asymmetry, development of the nervous system and the formation of cartilage and bone.

Specifications

Catalog Number	KC-3688
Cell Line Name	293T-CDH2 Cell Line
NCBI/UniProt Accession Number	NM_001792
Clone Number	3#
Host Cell Line	293T
Description	Stable 293T cell line expressing exogenous CDH2 gene
Quantity	One vial of frozen cells (≥2-10⁶/vial)
Stability	Stable in culture over a minimum of 10 passages
Application	Drug screening and biological assays
Freezing Medium	70% DMEM + 20% FBS + 10% DMSO
Propagation Medium	DMEM + 10% FBS + 1μg/mL Puromycin
Selection Marker	Puromycin
Morphology	Epithelial
Subculture	Split saturated culture 1:4-1:5 every 2-3 days; seed out at about 1-3 × 10⁵ cells/mL
Incubation	37 °C with 5% CO₂
Storage	Liquid nitrogen immediately upon receiving
Doubling Time	Approximately 30 hours
Mycoplasma Status	Negative
In Vivo Validation	NA

Cell Line Generation

293T-CDH2 cell line was generated using a lentiviral vector expressing the CDH2 sequence.

Characterization

Figure 1: Characterization of CDH2 overexpression in the 293T-CDH2 stable clone using FACS.

Figure 2: Characterization of CDH2 overexpressing in 293T-CDH2 stable clone using PCR sequencing.

Cell Resuscitation

Prewarm culture medium (DMEM supplemented with 10% FBS and 1μg/mL Puromycin)in a 37°C water bath.
Thaw the frozen vial in a 37°C water bath for 1-2 minutes.
Transfer the vial into biosafety cabinet, and wipe the surface with 70% ethanol.
Unscrew the top of the vial and transfer the cell suspension gently into a sterile centrifuge tube containing 9.0mL complete culture medium.
Spin at ~ 125 × g for 5-7 minutes at room temperature, and discard the supernatant without disturbing the pellet.
Resuspend cell pellet with the appropriate volume of complete medium and transfer the cell suspension into a T25 culture flask.
Incubate the flask at 37°C, 5% CO₂ incubator.
Split saturated culture 1:4-1:5 every 2-3 days; seed out at about 1-3 × 10⁵ cells/mL.

Cell Freezing

Prepare the freezing medium (70% DMEM + 20% FBS + 10% DMSO) fresh immediately before use.
Keep the freezing medium on ice and label cryovials.
Transfer cells to a sterile, conical centrifuge tube, and count the cells.
Centrifuge the cells at 250×g for 5 minutes at room temperature and carefully aspirate off the medium.
Resuspend the cells at a density of at least 3×10⁶ cells/mL in chilled freezing medium.
Aliquot 1 mL of the cell suspension into each cryovial.
Freeze cells in the CoolCell freezing container overnight in a -80°C freezer.
Transfer vials to liquid nitrogen for long-term storage.

References

Chung H, Jung H, Jho EH, Multhaupt HAB, Couchman JR, Oh ES. Keratinocytes negatively regulate the N-cadherin levels of melanoma cells via contact-mediated calcium regulation. Biochem Biophys Res Commun. 2018 Sep 5;503(2):615-620. doi: 10.1016/j.bbrc.2018.06.050. Epub 2018 Jun 14. PMID: 29902459.
He K, Yu P, Xing HY, Li Y, Tian Z, Wang M, Tang KJ, Rao Q. [Resistance of leukemia KG1a cells with positive N-cadherin in phase G(0) against killing activity of VP16]. Zhongguo Shi Yan Xue Ye Xue Za Zhi. 2011 Oct;19(5):1102-6. Chinese. PMID: 22040951.

Use License Agreement

Research Use Only.

Not for use in diagnostic procedures or therapeutic applications.

Redistribution of the cell line or its derivatives is prohibited without prior written permission from Kyinno Biotechnology.