KC-3900

293T-FCER1A Cell Line

34996

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Background of 293T-FCER1A Cell Line

FCER1A encodes the alpha subunit of the high-affinity IgE receptor (FcεRI), which is primarily expressed on mast cells and basophils. This receptor plays a critical role in allergic responses by binding to the Fc region of immunoglobulin E (IgE). Cross-linking of IgE-bound FcεRI by allergens triggers cell degranulation and release of inflammatory mediators such as histamine and cytokines. The FCER1A gene is located on chromosome 1q23.3, and genetic variations in this gene have been associated with atopic conditions, including asthma, eczema, and food allergies.

Specifications

Catalog Number	KC-3900
Cell Line Name	293T-FCER1A Cell Line
NCBI/UniProt Accession Number	NM_002001.4
Clone Number	1#
Host Cell Line	293T
Description	Stable 293T cell line expressing exogenous FCER1A gene.
Quantity	One vial of frozen cells (≥2-10⁶/vial)
Stability	Stable in culture over a minimum of 10 passages
Application	Drug screening and biological assays
Freezing Medium	70% DMEM+20% FBS+10% DMSO
Propagation Medium	DMEM+10% FBS+1μg/mL Puromycin
Selection Marker	Puromycin
Morphology	Epithelial
Subculture	Split saturated culture 1:4-1:8 every 2-3 days; seed out at about 1-3 × 10⁵ cells/mL
Incubation	37 °C with 5% CO₂
Storage	Liquid nitrogen immediately upon receiving
Doubling Time	Approximately 30 hours
Mycoplasma Status	Negative
In Vivo Validation	NA

Cell Line Generation

293T-FCER1A cell line was generated using lentivirus expressing FCER1A sequence.

Characterization

Figure 1. Characterization of FCER1A over-expression in the 293T-FCER1A stable clone using qPCR.

Figure 2. Characterization of 293T-FCER1A cell line stable clone using PCR sequencing.

Cell Resuscitation

Prewarm culture medium (DMEM supplemented with 10% FBS and 1μg/mL Puromycin)in a 37°C water bath.
Thaw the frozen vial in a 37°C water bath for 1-2 minutes.
Transfer the vial into biosafety cabinet, and wipe the surface with 70% ethanol.
Unscrew the top of the vial and transfer the cell suspension gently into a sterile centrifuge tube containing 9.0mL complete culture medium.
Spin at ~ 125 × g for 5-7 minutes at room temperature, and discard the supernatant without disturbing the pellet.
Resuspend cell pellet with the appropriate volume of complete medium and transfer the cell suspension into a T25 culture flask.
Incubate the flask at 37°C, 5% CO₂ incubator.
Split saturated culture 1:4-1:8 every 2-3 days; seed out at about 1-3 × 10⁵ cells/mL.

Cell Freezing

Prepare the freezing medium (70% DMEM + 20% FBS + 10% DMSO) fresh immediately before use.
Keep the freezing medium on ice and label cryovials.
Transfer cells to a sterile, conical centrifuge tube, and count the cells.
Centrifuge the cells at 250×g for 5 minutes at room temperature and carefully aspirate off the medium.
Resuspend the cells at a density of at least 3×10⁶ cells/mL in chilled freezing medium.
Aliquot 1 mL of the cell suspension into each cryovial.
Freeze cells in the CoolCell freezing container overnight in a -80°C freezer.
Transfer vials to liquid nitrogen for long-term storage.

References

Chen CM, Weidinger S, Klopp N, Sausenthaler S, Bischof W, Herbarth O, Bauer M, Borte M, Schaaf B, Lehmann I, Behrendt H, Krämer U, Berdel D, von Berg A, Bauer CP, Koletzko S, Illig T, Wichmann HE, Heinrich J; LISA and GINI Study Group. Common variants in FCER1A influence total serum IgE levels from cord blood up to six years of life. Allergy. 2009 Sep;64(9):1327-32. doi: 10.1111/j.1398-9995.2009.02005.x. Epub 2009 Feb 24. PMID: 19245427.

Use License Agreement

Research Use Only.

Not for use in diagnostic procedures or therapeutic applications.

Redistribution of the cell line or its derivatives is prohibited without prior written permission from Kyinno Biotechnology.