| Catalog Number | KC-4034 |
|---|---|
| Cell Line Name | 293T-STAT5-Luc2-TSLPR-IL7Ra-Cell-Line |
| Host Cell Line | 293T |
| Description | Stable 293T cell line expressing exogenous luciferase gene under the control of TSLPR-IL7Ra signaling pathway |
| Quantity | Two vials of frozen cells (≥2-106/vial) |
| Stability | Stable in culture over a minimum of 10 passages |
| Application | Cell model for monitoring TSLPR-IL7Ra signaling pathway |
| Freezing Medium | 70% DMEM+20% FBS+10% DMSO |
| Propagation Medium | DMEM+10% FBS +150μg/ml Hygromycin+1μg/ml Puromycin |
| Selection Marker | Hygromycin and Puromycin |
| Morphology | Epithelial |
| Subculture | Split saturated culture 1:4-1:8 every 2-3 days; seed out at about 1-3 × 105 cells/mL |
| Incubation | 37 °C with 5% CO2 |
| Storage | Liquid nitrogen immediately upon receiving |
| Doubling Time | Approximately 30 hours |
| Mycoplasma Status | Negative |
| In Vivo Validation | NA |
Figure 1. Characterization of TSLPR-IL7Ra over-expression in 293T-STAT5-Luc2-TSLPR-IL7Ra stable clones using FACS.
Figure 2. 293T-STAT5-Luc2-TSLPR-IL7Ra cell line was seeded into the 96-well plate, and treated with TSLP at a maximum concentration of 10μg/mL for 16 hours, then readout with Bright-Glo system.
Figure 3. 293T-STAT5-Luc2-TSLPR-IL7Ra cell line was seeded into the 96-well plate, then treated with TSLP (100ng/mL), incubated for 16 hours and readout with Bright-Glo system.
Figure 4. Characterization of 293T-STAT5-Luc2-TSLPR-IL7Ra cell line stable clone using PCR sequencing.
