KB-1363

SWA11-hIgG1

45570

Background of SWA11-hIgG1

CD24 is a highly glycosylated protein that is linked to the membrane via glycosylphosphatidylinositol (GPI)-anchor and is often highly expressed in carcinomas and correlates with poor prognosis. SWA11-hIgG1-CD24, is a recombinant IgG1 kappa, human monoclonal antibody produced in CHO cell culture, which selectively binds with high affinity to the extracellular domain of CD24 protein and can efficiently retard the growth of lung and ovarian carcinoma xenografts. Importantly, SWA11-hIgG1-CD24 treatment strongly influenced intratumoural cytokine milieu and increased infiltration of tumour tissue by macrophages.

Specifications

Catalog Number	KB-1363
Antibody Name	SWA11-hIgG1
Isotype	Human IgG1,kappa
Target	CD24
Species Reactivity	Human
Host Cell Line	EXPI CHO
Purification Method	Affinity purified
Concentration	>2 mg/mL
Formulation	50 mM sodium citrate,150mM NaCl,pH5.5
Purity	>95% by SDS-PAGE and SEC-HPLC
Validation	FACS
Endotoxin Level	<0.2 EU/mg as determined by the LAL method
Sterility	0.2μm filtered
Storage	It is recommended that the protein should be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Stability	Stable for twelve months from date of receipt when stored at -20°C to -80°C; Stored at 2-8°C for one month without detectable loss of activity.

Characterization

Application

References

Kay R, Rosten PM, Humphries RK (1991) CD24, a signal transducer modulating B cell activation responses, is a very short peptide with a glycosyl phosphatidylinositol membrane anchor. J Immunol 147: 1412–1416
Bretz NP, Salnikov AV, Perne C, Keller S, Wang X, Mierke CT, Fogel M, Erbe-Hofmann N, Schlange T, Moldenhauer G, Altevogt P (2012) CD24 controls Src/STAT3 activity in human tumors. Cell Mol Life Sci 69 (22): 3863–3879