KB-1475

FITC4420-hIgG1

45760

Background of FITC4420-hIgG1

Fluorescein isothiocyanate (FITC) is a small hapten that is commonly used as a fluorescent probe to label proteins, nucleic acid molecules, and drug tracers. FITC is stable and one anti-FITC antibody can bind two FITC molecules at the same time. The FITC-anti-FITC system can be used to improve the sensitivity of antigen-antibody reaction in immune detection. At the same time, the biological samples do not contain natural FITC, so the specificity of the system is also higher than that of the biotin-streptavidin system.

Specifications

Catalog Number	KB-1475
Antibody Name	FITC4420-hIgG1
Isotype	Human IgG1,kappa
Target	FITC
Species Reactivity	Human
Host Cell Line	EXPI CHO
Purification Method	Affinity purified
Concentration	>2 mg/mL
Formulation	50 mM sodium citrate,150mM NaCl,pH5.5
Purity	>95% by SDS-PAGE and SEC-HPLC
Validation	ELISA
Endotoxin Level	<0.2 EU/mg as determined by the LAL method
Sterility	0.2μm filtered
Storage	It is recommended that the protein should be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Stability	Stable for twelve months from date of receipt when stored at -20°C to -80°C; Stored at 2-8°C for one month without detectable loss of activity.

Characterization

Application

References

D. Samuel, R.J. Patt, R.A. Abuknesha, et al. A sensitive method of detecting proteins on dot and Western blots using a monoclonal antibody to FITC. Journal of Immunological Methods. 1988,107(2):217-224.
Byung Soo Kim , Jae Min Oh, Kyung Sook Kim, Kwang Soo Seo, Jae Song Cho, Gilson Khang, Hai Bang Lee, Kinam Park, Moon Suk Kim. BSA-FITC-loaded microcapsules for in vivo delivery.Biomaterials. 2009 Feb;30(5):902-9.