KB-1076

1452C11-mIgG2a

46626

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Background of 1452C11-mIgG2a

The 145-2C11 antibody is responsive to the 20 kDa CD3εsubunit present in the T cell receptor (TCR)/CD3 complex of mice. This subunit is found on the surface of mature T cells, NKT cells, and occasionally on thymocytes. Additionally, CD3 is expressed by a majority of T cell neoplasms. The CD3 complex consists of CD3 γ,δ,ε,η, andζsubunits, which assemble non-covalently with the TCR. This complex plays a crucial role in transmitting antigen recognition signals to the cytoplasm of T cells and regulates the expression of the TCR on the cell surface.

Specifications

Catalog Number	KB-1076
Antibody Name	1452C11-mIgG2a
Isotype	Mouse IgG2a,kappa
FC Muations	Wild Type
Target	Mouse-CD3
Species Reactivity	Mouse
Host Cell Line	EXPI CHO
Purification Method	Affinity purified
Concentration	>2 mg/mL
Formulation	50 mM sodium citrate,150mM NaCl,pH5.5
Purity	>95% by SDS-PAGE and SEC-HPLC
Validation	FACS
Endotoxin Level	<0.2 EU/mg as determined by the LAL method
Sterility	0.2μm filtered
Storage	It is recommended that the protein should be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Stability	Stable for twelve months from date of receipt when stored at -20°C to -80°C; Stored at 2-8°C for one month without detectable loss of activity.

Characterization

Application

References

Hooks MA, Wade CS, Millikan WJ. Muromonab CD-3: a review of its pharmacology, pharmacokinetics, and clinical use in transplantation. Pharmacotherapy 11(1), 26–37 (1991).
Chatenoud L, Baudrihaye MF, Chkoff N, Kreis H, Goldstein G, Bach J-F. Restriction of the human in vivo immune response against the mouse monoclonal antibody OKT3. J. Immunol. 137(3), 830–838 (1986).