KB-1233

OKT3

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Background of OKT3

OKT3 is a monoclonal antibody targeted at the CD3 receptor located on the surface of T cells. Being approved by the FDA in 1985, it is also an immunosuppressant drug given to reduce acute rejection in patients with organ transplants, making it the first monoclonal antibody to be approved anywhere as a drug for humans.

Specifications

Catalog NumberKB-1233
Antibody NameOKT3
IsotypeMouse IgG2a, kappa
FC MuationsWild Type
TargetCD3
Species ReactivityHuman
Host Cell LineEXPI CHO
Purification MethodAffinity purified
Concentration>2 mg/mL
Formulation50 mM sodium citrate,150mM NaCl,pH5.5
Purity>95% by SDS-PAGE and SEC-HPLC
ValidationELISA
Endotoxin Level<0.2 EU/mg as determined by the LAL method
Sterility0.2μm filtered
StorageIt is recommended that the protein should be aliquoted for optimal storage.
Avoid repeated freeze-thaw cycles.
StabilityStable for twelve months from date of receipt when stored at -20°C to -80°C;
Stored at 2-8°C for one month without detectable loss of activity.

Characterization

Application

References

  1. Pauline Wimberger Wei Xiang &Doris Mayr et al. Efficient tumor cell lysis by autologous, tumor-resident T lymphocytes in primary ovarian cancer samples by an EP-CAM-/CD3-bispecific antibody. IJC. Volume105, Issue2(2003).
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