KC-5416

293T-rat-SLC39A6-Cell-Line

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Background of 293T-rat-SLC39A6-Cell-Line

SLC39A6 (Solute Carrier Family 39 Member 6) is a Protein Coding gene. Diseases associated with SLC39A6 include Acrodermatitis Enteropathica and Breast Cancer. Among its related pathways are Metal ion SLC transporters and Transport of inorganic cations/anions and amino acids/oligopeptides. Gene Ontology (GO) annotations related to this gene include metal ion transmembrane transporter activity and zinc ion transmembrane transporter activity. An important paralog of this gene is SLC39A10

Specifications

Catalog Number	KC-5416
Cell Line Name	293T-rat-SLC39A6-Cell-Line
Clone Number	2#
Host Cell Line	293T
Description	Stable 293T clone expressing exogenous rat-SLC39A6 gene
Quantity	Two vials of frozen cells (≥2-10⁶/vial)
Stability	Stable in culture over a minimum of 10 passages
Application	Drug screening and biological assays
Freezing Medium	70% DMEM + 20% FBS + 10% DMSO
Propagation Medium	DMEM + 10% FBS + 1μg/mL Puromycin
Selection Marker	Puromycin
Morphology	Epithelial
Subculture	Split saturated culture 1:4-1:8 every 2-3 days; seed out at about 1-3 × 10⁵ cells/mL
Incubation	37 °C with 5% CO₂
Storage	Liquid nitrogen immediately upon receiving
Doubling Time	Approximately 30 hours
Mycoplasma Status	Negative

Cell Line Generation

293T-rat-SLC39A6-cell-line was generated using a lentiviral vector expressing the rat-SLC39A6 sequence.

Characterization

Figure 1: Characterization of rat-SLC39A6 overexpression in the 293T-rat-SLC39A6 stable clone using qPCR.

Figure 2: Characterization of rat-SLC39A6 in the 293T-rat-SLC39A6 stable clone using PCR sequencing.

Cell Resuscitation

1. Prewarm culture medium (DMEM supplemented with 10% FBS and 1μg/mL puromycin)in a 37°C water bath.
2. Thaw the frozen vial in a 37°C water bath for 1-2 minutes.
3. Transfer the vial into biosafety cabinet, and wipe the surface with 70% ethanol.
4. Unscrew the top of the vial and transfer the cell suspension gently into a sterile centrifuge tube containing 9.0mL complete culture medium.
5. Spin at ~ 125 × g for 5-7 minutes at room temperature, and discard the supernatant without disturbing the pellet.
6. Resuspend cell pellet with the appropriate volume of complete medium and transfer the cell suspension into a T25 culture flask.
7. Incubate the flask at 37°C, 5% CO₂ incubator.
8. Split saturated culture 1:4-1:8 every 2-3 days; seed out at about 1-3 × 10⁵ cells/mL.

Cell Freezing

1. Prepare the freezing medium (70% DMEM + 20% FBS + 10% DMSO) fresh immediately before use.
2. Keep the freezing medium on ice and label cryovials.
3. Transfer cells to a sterile, conical centrifuge tube, and count the cells.
4. Centrifuge the cells at 250×g for 5 minutes at room temperature and carefully aspirate off the medium.
5. Resuspend the cells at a density of at least 3×10⁶ cells/mL in chilled freezing medium.
6. Aliquot 1 mL of the cell suspension into each cryovial.
7. Freeze cells in the CoolCell freezing container overnight in a -80°C freezer.
8. Transfer vials to liquid nitrogen for long-term storage.

References

1.Liu X, Liu W, Wu Y, Wang Y, Jiang Q, Li Y, Li H, Hao L. Investigation of the cytotoxic effects and mechanisms of the SLC39A6-targeting ADC drug BRY812 in CRC. Sci Rep. 2025 May 25;15(1):18275. doi: 10.1038/s41598-025-03713-1. PMID: 40414981; PMCID: PMC12104431.
2.Ghaderi H, Noormohammadi Z, Habibi-Anbouhi M, Kazemi-Lomedasht F, Behdani M. Development of camelid monoclonal nanobody against SLC39A6 zinc transporter protein. Iran J Basic Med Sci. 2021 Dec;24(12):1726-1733. doi: 10.22038/IJBMS.2021.58542.13003. PMID: 35432806; PMCID: PMC8976899.