KC-2292-DW

MC38-GDF15 Cell Line

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Background of MC38-GDF15 Cell Line

GDF15, also named MIC-1, is a member of TGF-beta superfamily proteins, and plays an important role in tumorigenesis and metastasis. the expression of GDF-15 is dramatically increased in many types of cancers, such as colorectal, breast, and prostate. GDF-15 also mediates cancer-induced anorexia and weight loss through the modulation of neuronal pathways important in the regulation of appetite and energy homeostasis.

Specifications

Catalog Number	KC-2292-DW
Cell Line Name	MC38-GDF15 Cell Line
NCBI/UniProt Accession Number	NM_004864.4
Clone Number	5#
Host Cell Line	MC38
Description	Stable MC38-GDF15 cell line expressing exogenous GDF15 gene
Quantity	One vial of frozen cells (≥2-10⁶/vial)
Stability	Stable in culture over a minimum of 10 passages
Application	Drug screening and biological assays
Freezing Medium	70% DMEM+20% FBS+10% DMSO
Propagation Medium	DMEM+10% FBS+5μg/mL Puromycin
Selection Marker	Puromycin
Morphology	Epithelial
Subculture	Split saturated culture 1:4-1:10 every 2-3 days; seed out at about 1-3 × 10⁵ cells/mL
Incubation	37 °C with 5% CO₂
Storage	Liquid nitrogen immediately upon receiving
Doubling Time	Approximately 20 hours
Mycoplasma Status	Negative

Cell Line Generation

MC38-GDF15 cell line was generated using lentivirus expressing GDF15 sequence.

Characterization

Figure 1. Characterization of GDF15 over-expression in the MC38-GDF15 stable clone using Elisa.

Figure 2:Validation of in vivo tumorigenicity of MC38 cells stably expressing GDF15 via subcutaneous implantation in C57BL/6J mice, with tumor growth monitored by measuring volume (V=0.5×length×width²) and body weight.

Cell Resuscitation

Prewarm culture medium (DMEM+10% FBS+5μg/mL Puromycin)in a 37°C water bath.
Thaw the frozen vial in a 37°C water bath for 1-2 minutes.
Transfer the vial into biosafety cabinet, and wipe the surface with 70% ethanol.
Unscrew the top of the vial and transfer the cell suspension gently into a sterile centrifuge tube containing 9.0mL complete culture medium.
Spin at ~ 125 × g for 5-7 minutes at room temperature, and discard the supernatant without disturbing the pellet.
Resuspend cell pellet with the appropriate volume of complete medium and transfer the cell suspension into a T25 culture flask.
Incubate the flask at 37°C, 5% CO₂ incubator.
Split saturated culture 1:4-1:10 every 2-3 days; seed out at about 1-3 × 10⁵ cells/mL.

Cell Freezing

Prepare the freezing medium (70% DMEM + 20% FBS + 10% DMSO) fresh immediately before use.
Keep the freezing medium on ice and label cryovials.
Transfer cells to a sterile, conical centrifuge tube, and count the cells.
Centrifuge the cells at 250×g for 5 minutes at room temperature and carefully aspirate off the medium.
Resuspend the cells at a density of at least 3×10⁶ cells/mL in chilled freezing medium.
Aliquot 1 mL of the cell suspension into each cryovial.
Freeze cells in the CoolCell freezing container overnight in a -80°C freezer.
Transfer vials to liquid nitrogen for long-term storage.

References

GDF15, an update of the physiological and pathological roles it plays: a review.Artin Assadi , Azadeh Zahabi&Robert A Hart .Affiliations expand PMID: 32936319
Secreted and Cell Surface Genes Expressed in Benign and Malignant Colorectal Tumors. Advances in Brief DOI: Published October 2001 doi:10.1006/geno.1998.5281. PMID 9615235.
The Transforming Growth Factor-β Superfamily Member Growth-Differentiation Factor-15 Protects the Heart From Ischemia/Reperfusion Injury.Circulation Research. 2006;98:351-360.Originally published5 Jan 2006.

Use License Agreement

Research Use Only.

Not for use in diagnostic procedures or therapeutic applications.

Redistribution of the cell line or its derivatives is prohibited without prior written permission from Kyinno Biotechnology.