KC-5998

293T-MRC1 Cell Line

64695

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Background of 293T-MRC1 Cell Line

MRC1 (Mannose Receptor C-Type 1) is a Protein Coding gene. MRC1 encodes mannose receptor C-type 1, a C-type lectin receptor that mediates recognition and endocytosis of glycoproteins on macrophages. It binds mannose, fucose, and N-acetylglucosamine on glycoproteins and microbial surfaces, linking carbohydrate recognition to clearance functions in innate immunity. Pathogenic or disease-associated relevance for MRC1 has been reported in immune and infectious disease contexts. It is linked to Dengue, Hepatitis B, and broader pathogen clearance biology through its recognition of microbial carbohydrates and envelope proteins.

Specifications

Catalog Number	KC-5998
Cell Line Name	293T-MRC1 Cell Line
NCBI/UniProt Accession Number	NM_002438
Clone Number	6-8#
Host Cell Line	293T
Description	Stable 293T cell line expressing exogenous human MRC1 gene
Quantity	Two vials of frozen cells (≥2-10⁶/vial)
Stability	Stable in culture over a minimum of 10 passages
Application	Drug screening and biological assays
Freezing Medium	70% DMEM+20% FBS+10% DMSO
Propagation Medium	DMEM+10% FBS +1μg/mL Puromycin
Selection Marker	Puromycin
Morphology	Epithelial-like
Subculture	Split saturated culture 1:4-1:5 every 2-3 days
Incubation	37 °C with 5% CO₂
Storage	Liquid nitrogen immediately upon receiving
Doubling Time	Approximately 30 hours
Mycoplasma Status	Negative

Cell Line Generation

293T-MRC1 cell line was generated using a lentiviral vector expressing the human MRC1 sequence.

Characterization

Figure 1: Characterization of human MRC1 overexpression in the 293T-MRC1 stable clone using FACS.

Figure 2: Characterization of human MRC1 overexpression in the 293T-MRC1 stable clone using QPCR.

Figure 3: Characterization of human MRC1 in the 293T-MRC1 stable clone using PCR sequencing.

Cell Resuscitation

Pre-warm complete culture medium (DMEM+10% FBS +1μg/mL Puromycin) in a 37°C water bath.
Rapidly thaw the cryovial in a 37°C water bath for 1-2 minutes with gentle agitation.
Transfer the vial to a biosafety cabinet, and disinfect the exterior with 70% ethanol.
Aseptically transfer the cell suspension dropwise into a sterile centrifuge tube containing 9.0 mL of pre-warmed complete medium.
Centrifuge at approximately 125 × g for 5–7 minutes at room temperature, carefully aspirate the supernatant without disturbing the cell pellet.
Gently resuspend the pellet in an appropriate volume of complete medium and transfer the suspension into a T25 flask.
Incubate the flask in a 37°C in a humidified 5% CO₂ incubator.
Assess cell viability and morphology after 24 hours. If cells appear healthy, replace the medium with fresh medium supplemented with the appropriate selective antibiotic.
Subculture the cells at a ratio of 1:4-1:5 every 2-3 days upon reaching 80%–90% confluency.

Cell Freezing

Prepare the freezing medium (70% basal medium, 20% FBS and 10% DMSO) freshly before use.
Pre-chill the freezing medium on ice and label the cryovials accordingly.
Transfer the cell suspension to a sterile conical tube and perform a cell count to determine total viability and density.
Centrifuge the cells at 250×g for 5 minutes at room temperature; carefully aspirate the supernatant.
Gently resuspend the cell pellet in chilled freezing medium, ensuring a minimum cell density of 3×10⁶ cells/mL.
Aliquot 1 mL of the cell suspension into each pre-labeled cryovial.
Place the cryovials into a CoolCell® container and store at -80°C overnight for controlled-rate cooling.
Transfer the cryovials to the liquid nitrogen for long-term storage the following day.

References

1. Masai H, Yang CC, Matsumoto S. Mrc1/Claspin: a new role for regulation of origin firing. Curr Genet. 2017 Oct;63(5):813-818. doi: 10.1007/s00294-017-0690-y. Epub 2017 Mar 29. PMID: 28357499.
2. Toda T, Fang Y, Shan CM, Hua X, Kim JK, Tang LC, Jovanovic M, Tong L, Qiao F, Zhang Z, Jia S. Mrc1 regulates parental histone segregation and heterochromatin inheritance. Mol Cell. 2024 Sep 5;84(17):3223-3236.e4. doi: 10.1016/j.molcel.2024.07.002. Epub 2024 Aug 1. PMID: 39094566; PMCID: PMC11414769.
3. Wu Y, Yang S, Ma J, Chen Z, Song G, Rao D, Cheng Y, Huang S, Liu Y, Jiang S, Liu J, Huang X, Wang X, Qiu S, Xu J, Xi R, Bai F, Zhou J, Fan J, Zhang X, Gao Q. Spatiotemporal Immune Landscape of Colorectal Cancer Liver Metastasis at Single-Cell Level. Cancer Discov. 2022 Jan;12(1):134-153. doi: 10.1158/2159-8290.CD-21-0316. Epub 2021 Aug 20. PMID: 34417225.
4. Liang BE, Long LS, Wu XY, Huang MY, Lai Y, Yuan X, Wang MH, Li M, Zheng QQ, Zhang HL, Chen MC, Liu ZD, Geng X, Lyu QQ, Wang WD, Liu QH, Liu WZ, Li CL. Alginate oligosaccharide prevents renal ischemia-reperfusion injury in rats via MRC1-mediated pathway. Acta Pharmacol Sin. 2025 Sep;46(9):2450-2467. doi: 10.1038/s41401-025-01545-3. Epub 2025 Apr 22. PMID: 40263568; PMCID: PMC12373917.

Use License Agreement

Research Use Only.

Not for use in diagnostic procedures or therapeutic applications.

Redistribution of the cell line or its derivatives is prohibited without prior written permission from Kyinno Biotechnology.