KB-1076

1452C11-mIgG2a

46626

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Background of 1452C11-mIgG2a

The 145-2C11 antibody is responsive to the 20 kDa CD3εsubunit present in the T cell receptor (TCR)/CD3 complex of mice. This subunit is found on the surface of mature T cells, NKT cells, and occasionally on thymocytes. Additionally, CD3 is expressed by a majority of T cell neoplasms. The CD3 complex consists of CD3 γ,δ,ε,η, andζsubunits, which assemble non-covalently with the TCR. This complex plays a crucial role in transmitting antigen recognition signals to the cytoplasm of T cells and regulates the expression of the TCR on the cell surface.

Specifications

Catalog Number	KB-1076
Antibody Name	1452C11-mIgG2a
Isotype	Mouse IgG2a,kappa
FC Muations	Wild Type
Target	Mouse CD3
Species Reactivity	Mouse
Host Cell Line	EXPI CHO
Purification Method	Affinity purified
Concentration	>2 mg/mL
Formulation	50 mM sodium citrate,150mM NaCl,pH5.5
Purity	>95% by SDS-PAGE and SEC-HPLC
Validation	FACS
Endotoxin Level	<0.2 EU/mg as determined by the LAL method
Sterility	0.2μm filtered
Storage	It is recommended that the protein should be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Stability	Stable for twelve months from date of receipt when stored at -20°C to -80°C; Stored at 2-8°C for one month without detectable loss of activity.

Characterization

Application

References

Hooks MA, Wade CS, Millikan WJ. Muromonab CD-3: a review of its pharmacology, pharmacokinetics, and clinical use in transplantation. Pharmacotherapy 11(1), 26–37 (1991).
Chatenoud L, Baudrihaye MF, Chkoff N, Kreis H, Goldstein G, Bach J-F. Restriction of the human in vivo immune response against the mouse monoclonal antibody OKT3. J. Immunol. 137(3), 830–838 (1986).

Use License Agreement

Research Use Only.

Not for use in diagnostic procedures or therapeutic applications.