KC-1799

293T-cyno-IL11RA-Cell-Line

22825

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Background of 293T-cyno-IL11RA-Cell-Line

IL11RA is a glycoprotein that consists of 11 receptors. IL11RA receptor exists widely on the cell surface, binds to a variety of cytokines and growth factor receptors, mediates cell signaling pathways, and influences cell growth and metabolic processes. It plays an important role in inflammation, immune response and tumorigenesis. IL11RA can be used as a biomarker of tumor tissue for tumor diagnosis and monitoring.

Specifications

Catalog Number	KC-1799
Cell Line Name	293T-cyno-IL11RA-Cell-Line
Host Cell Line	293T
Description	Stable HEK293T clone expressing exogenous cyno IL11RA gene
Quantity	Two vials of frozen cells (≥2-10⁶/vial)
Stability	Stable in culture over a minimum of 10 passages
Application	Drug screening and biological assays
Freezing Medium	70% DMEM + 20% FBS + 10% DMSO
Propagation Medium	DMEM + 10% FBS + 1μg/mL Puromycin
Selection Marker	Puromycin
Morphology	Epithelial
Subculture	Split saturated culture 1:4-1:8 every 2-3 days; seed out at about 1-3 × 10⁵ cells/mL
Incubation	37 °C with 5% CO₂
Storage	Liquid nitrogen immediately upon receiving
Doubling Time	Approximately 30 hours
Mycoplasma Status	Negative
In Vivo Validation	NA

Cell Line Generation

293T cyno IL11RA cell line was generated using a lentiviral vector expressing the cyno IL11RA sequence.

Characterization

Figure 1: Characterization of cyno IL11RA overexpression in the 293T cyno IL11RA stable clone using FACS.

Cell Resuscitation

Prewarm culture medium (DMEM supplemented with 10% FBS and 1μg/mL puromycin)in a 37°C water bath.
Thaw the frozen vial in a 37°C water bath for 1-2 minutes.
Transfer the vial into biosafety cabinet, and wipe the surface with 70% ethanol.
Unscrew the top of the vial and transfer the cell suspension gently into a sterile centrifuge tube containing 9.0mL complete culture medium.
Spin at ~ 125 × g for 5-7 minutes at room temperature, and discard the supernatant without disturbing the pellet.
Resuspend cell pellet with the appropriate volume of complete medium and transfer the cell suspension into a T25 culture flask.
Incubate the flask at 37°C, 5% CO₂ incubator.
Split saturated culture 1:4-1:8 every 2-3 days; seed out at about 1-3 × 10⁵ cells/mL.

Cell Freezing

Prepare the freezing medium (70% DMEM + 20% FBS + 10% DMSO) fresh immediately before use.
Keep the freezing medium on ice and label cryovials.
Transfer cells to a sterile, conical centrifuge tube, and count the cells.
Centrifuge the cells at 250×g for 5 minutes at room temperature and carefully aspirate off the medium.
Resuspend the cells at a density of at least 3×10⁶ cells/mL in chilled freezing medium.
Aliquot 1 mL of the cell suspension into each cryovial.
Freeze cells in the CoolCell freezing container overnight in a -80°C freezer.
Transfer vials to liquid nitrogen for long-term storage

References

Suzuki S, Sasaki K, Fukazawa T, Kubo T. Xenopus laevis il11ra.L is an experimentally proven interleukin-11 receptor component that is required for tadpole tail regeneration. Sci Rep. 2022 Feb 3;12(1):1903. doi: 10.1038/s41598-022-05954-w. PMID: 35115663; PMCID: PMC8814168.
Balakrishnan L, Soman S, Patil YB, Advani J, Thomas JK, Desai DV, Kulkarni-Kale U, Harsha HC, Prasad TS, Raju R, Pandey A, Dimitriadis E, Chatterjee A. IL-11/IL11RA receptor mediated signaling: a web accessible knowledgebase. Cell Commun Adhes. 2013 Aug;20(3-4):81-6. doi: 10.3109/15419061.2013.791683. Epub 2013 Apr 30. PMID: 23631681.