KC-6384

293T-FCGR1A Cell Line

66237

Home » 293T-FCGR1A Cell Line

Background of 293T-FCGR1A Cell Line

FCGR1A encodes the high-affinity IgG receptor CD64, expressed on antigen-presenting cells, mediating ADCP, ADCC, and immune complex clearance, thus bridging innate and adaptive immunity. Recent studies show FCGR1A overexpression in various cancers, including ovarian, cervical, renal, and melanoma, where it promotes migration, invasion, and EMT via LSP1 regulation, correlating with advanced stage, lymph node metastasis, and poor prognosis. In rheumatoid arthritis, monocytic FCGR1A upregulation correlates with disease activity; genetic variants affect CD64 phagocytosis and cytokine production, associating with sarcoidosis susceptibility and severity. Collectively, FCGR1A overexpression plays pivotal roles in tumor progression and immunoinflammation, positioning it as a potential diagnostic, prognostic, and immunotherapeutic target.

Specifications

Catalog Number	KC-6384
Cell Line Name	293T-FCGR1A Cell Line
NCBI/UniProt Accession Number	NM_010186.5
Clone Number	8#
Host Cell Line	293T
Description	Stable 293T clone expressing exogenous FCGR1A gene
Quantity	Two vials of frozen cells (≥2-10⁶/vial)
Stability	Stable in culture over a minimum of 10 passages
Application	Drug screening and biological assays
Freezing Medium	70% DMEM + 20% FBS + 10% DMSO
Propagation Medium	DMEM + 10% FBS + 1μg/mL Puromycin
Selection Marker	Puromycin
Morphology	Fibroblastoid cells growing as a monolayer
Subculture	Split the saturated culture at a ratio of 1:4-1:5 every 2-3 days; seed out at about 1-3 x 10⁵ cells/mL
Incubation	37 °C with 5% CO₂
Storage	Liquid nitrogen immediately upon receiving
Doubling Time	Approximately 30 hours
Mycoplasma Status	Negative

Cell Line Generation

293T-FCGR1A cell line was generated using a lentiviral vector expressing the human FCGR1A sequence.

Characterization

Cell Resuscitation

Prewarm culture medium (DMEM+10% FBS+1μg/mL Puromycin)in a 37°C water bath.
Thaw the frozen vial in a 37°C water bath for 1-2 minutes.
Transfer the vial into biosafety cabinet, and wipe the surface with 70% ethanol.
Unscrew the top of the vial and transfer the cell suspension gently into a sterile centrifuge tube containing 9.0mL complete culture medium.
Spin at ~ 125 × g for 5-7 minutes at room temperature, and discard the supernatant without disturbing the pellet.
Resuspend cell pellet with the appropriate volume of complete medium and transfer the cell suspension into a T25 culture flask.
Incubate the flask at 37°C, 5% CO₂ incubator.
Split saturated culture 1:4-1:5 every 2-3 days; seed out at about 1-3 × 10⁵ cells/mL.

Cell Freezing

Prepare the freezing medium (70% DMEM + 20% FBS + 10% DMSO) fresh immediately before use.
Keep the freezing medium on ice and label cryovials.
Transfer cells to a sterile, conical centrifuge tube, and count the cells.
Centrifuge the cells at 250×g for 5 minutes at room temperature and carefully aspirate off the medium.
Resuspend the cells at a density of at least 3×10⁶ cells/mL in chilled freezing medium.
Aliquot 1 mL of the cell suspension into each cryovial.
Freeze cells in the CoolCell freezing container overnight in a -80°C freezer.
Transfer vials to liquid nitrogen for long-term storage.

References

Qi Y, Zhu W, Mo K, Jiang H. CRISPR/Cas9-based genome-wide screening for metastasis ability identifies FCGR1A regulating the metastatic process of ovarian cancer by targeting LSP1. J Cancer Res Clin Oncol. 2024 Jun 15;150(6):306. doi: 10.1007/s00432-024-05837-9. PMID: 38879666; PMCID: PMC11180010.

Use License Agreement

Research Use Only.

Not for use in diagnostic procedures or therapeutic applications.

Redistribution of the cell line or its derivatives is prohibited without prior written permission from Kyinno Biotechnology.