KC-1592

293T-FOLR1-Tet-on-TRPV6-Cell-Line

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Home » 细胞系 » 293T-FOLR1-Tet-on-TRPV6-Cell-Line

Background of 293T-FOLR1-Tet-on-TRPV6-Cell-Line

Specifications

Catalog NumberKC-1592
Cell Line Name293T-FOLR1-Tet-on-TRPV6-Cell-Line
Host Cell LineHuman HEK293T cell line
DescriptionStable HEK293T clone expressing exogenous human FOLR1 and TRPV6 under the control of tet-on system
QuantityTwo vials of frozen cells (≥2-106/vial)
StabilityStable in culture over a minimum of 10 passages
ApplicationDrug screening and biological assays
Freezing Medium70% DMEM+20% FBS+10% DMSO
Propagation MediumDMEM+10%FBS+1µg/mL Puromycin
Selection MarkerPuromycin
MorphologyEpithelial
SubcultureSplit saturated culture 1:3-1:6 every 3 days; seed out at about 1-3 × 105 cells/mL
Incubation37 °C with 5% CO2
StorageLiquid nitrogen immediately upon receiving
Doubling TimeApproximately 30 hours
Mycoplasma StatusNegative
In Vivo ValidationNA

Cell Line Generation

Characterization

Cell Resuscitation

Cell Freezing

References

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