| Catalog Number | KC-0222 |
|---|---|
| Cell Line Name | 293T-NFκB-Luc2-CD40 Cell Line |
| Host Cell Line | 293T-NFκB-Luc2 |
| Description | Stable Jurkat cell line expressing exogenous luciferase under the control of NFκB responsive element and CD40 fusion sequence. |
| Quantity | One vial of frozen cells (≥2-106/vial) |
| Stability | Stable in culture over a minimum of 10 passages |
| Application | Drug screening and biological assays |
| Freezing Medium | 70% DMEM + 20% FBS + 10% DMSO |
| Propagation Medium | DMEM + 10% FBS + 0.5μg/mL puromycin |
| Selection Marker | Puromycin |
| Morphology | Lymphoblast |
| Subculture | Split saturated culture 1:4-1:5 every 2-3 days; seed out at about 1 × 105 cells/mL |
| Incubation | 37 °C with 5% CO2 |
| Storage | Liquid nitrogen immediately upon receiving |
| Doubling Time | Approximately 30 hours |
| Mycoplasma Status | Negative |
Figure 1: Characterization of CD40 overexpressing in 293T-NFκB-Luc2 stable clones using FACS.
Figure 2: 293T-NFκB-Luc2-CD40 cell were seeded into 96-well plates, treated with Selicrelumab in different concentrations for 6 hours, and then read out using Bright-Glo Detection System.
Figure 3: 293T-NFκB-Luc2-CD40 cell were seeded into 96-well plates, treated with Recombinant Human CD40 Ligand/TNFSF5 in different concentrations for 6 hours, and then read out using Bright-Glo Detection System.
Figure 4: 293T-NFκB-Luc2-CD40 cells were seeded into 96-well plates, treated with COP-7657-hIgG1(Cat#KB-1587, Kyinno) or APB-A1-hlgG1(Cat#KB-1590, Kyinno) in different concentrations for 1 hours, and then treated with Recombinant Human CD40 Ligand/TNFSF5 in the concentration of 0.2μg/mL for 6 hours, and then read out using Bright-Glo Detection System."
