| Catalog Number | KC-4182 |
|---|---|
| Cell Line Name | 293T-SMAD-Luc2-ACVR2A-ACVR2B-KO-Cell-Line |
| NCBI/UniProt Accession Number | NA |
| Clone Number | 3B3 |
| Host Cell Line | 293T-ACVR2A-ACVR2B-KO |
| Description | Stable 293T cell line expressing exogenous luciferase gene under the control of SMAD transcription factor with human ACVR2A and ACVR2B gene knockout. |
| Quantity | One vial of frozen cells (≥2-106/vial) |
| Stability | Stable in culture over a minimum of 10 passages |
| Application | Drug screening and biological assays |
| Freezing Medium | 70% DMEM+20% FBS+10% DMSO |
| Propagation Medium | DMEM+10% FBS +150μg/mL Hygromycin B |
| Selection Marker | Hygromycin B |
| Morphology | Epithelial |
| Subculture | Split saturated culture 1:4-1:8 every 2-3 days |
| Incubation | 37 °C with 5% CO2 |
| Storage | Liquid nitrogen immediately upon receiving |
| Doubling Time | Approximately 30 hours |
| Mycoplasma Status | Negative |
| In Vivo Validation | NA |
Figure 1. 293T-SMAD-Luc2-ACVR2A-ACVR2B-KO cell line was seeded into the 96-well plate, and treated with TGFβ1 and Activin at a maximum concentration of 3.16ng/mL and 100ng/mL for 6 hours, then readout with Bright-Glo system.
Figure 2: 293T-SMAD-Luc2-ACVR2A-ACVR2B-KO cells were seeded into 96-well plates, treated with Activin A for 16 hours, and then read out using Bright-Glo Detection System.
