| Catalog Number | KC-2612 |
|---|---|
| Cell Line Name | 293T-STAT3-Luc2 Cell Line |
| Host Cell Line | Human HEK293T cell line |
| Description | Stable 293T cell line expressing exogenous luciferase gene under the control of STAT3 signaling pathway. |
| Quantity | Two vials of frozen cells (≥2-106/vial) |
| Stability | Stable in culture over a minimum of 10 passages |
| Application | Drug screening and biological assays |
| Freezing Medium | 70% DMEM+20% FBS+10% DMSO |
| Propagation Medium | DMEM+10%FBS+100μg/mL Hygromycin |
| Selection Marker | HygromycinB |
| Morphology | Epithelial |
| Subculture | Split saturated culture 1:3-1:6 every 3 days; seed out at about 1-3 × 105 cells/mL |
| Incubation | 37 °C with 5% CO2 |
| Storage | Liquid nitrogen immediately upon receiving |
| Doubling Time | Approximately 30 hours |
| Mycoplasma Status | Negative |
| In Vivo Validation | NA |
Figure 1. 293T-STAT3-Luc2 cell line was seeded into the 96-well plate, and treated with LIF at a maximum concentration of 100ng/ml diluted 3.16-fold for 6 hours, then readout with Bright-Glo system.
Figure 2. 293T-STAT3-Luc2 cell line was seeded into the 96-well plate, and treated with IL11 at a maximum concentration of 1000ng/ml diluted 3.16-fold for 6 hours, then readout with Bright-Glo system.
Figure 3. 293T-STAT3-Luc2 cell line was seeded into the 96-well plate, and treated with IL-6 at a maximum concentration of 100ng/ml diluted 3.16-fold for 6 hours, then readout with Bright-Glo system.
Figure 4. 293T-STAT3-Luc2 cell line was seeded into the 96-well plate, and treated with OSM at a maximum concentration of 316ng/ml diluted 3.16-fold for 6 hours, then readout with Bright-Glo system.
Figure 5. 293T-STAT3-Luc2 cell line was seeded into the 96-well plate, and treated with napabucasin at a maximum concentration of 10μM for 0.5 hours, then treated with IL-6 at a maximum concentration of 10ng/ml for 6 hours, then readout with Bright-Glo system.
