KB-1160

29F1A12-mIgG1-D265A

12165

Home » Antibodies » 29F1A12-mIgG1-D265A

Background of 29F1A12-mIgG1-D265A

CD279 is a cell surface protein predominantly expressed on T cells. Its ligands CD273 and CD274 are expressed on antigen-presenting cells and tumors. CD279 has been shown to act as an important immune check point by inhibiting CD8 T cell activation, and antibodies against CD279 enhance T cell-mediated cytotoxic function. Increases in the expression of programmed cell death protein 1, also known as PD-1 (CD279), and its ligand PD-L1 (CD274), mainly in differentiated M0 (CD68+CD11b+) macrophages.

Specifications

Catalog Number	KB-1160
Antibody Name	29F1A12-mIgG1-D265A
Isotype	Mouse-IgG1-D265A, kappa
Target	Mouse-PD1
Species Reactivity	Mouse
Host Cell Line	EXPI-CHO
Purification Method	Affinity purified
Concentration	>2mg/mL
Formulation	20mM sodium citrate,150mM NaCl, pH5.5
Purity	>95% by SDS-PAGE and SEC-SEC-HPLC
Validation	SDS-PAGE \| SEC-HPLC \| ELISA
Endotoxin Level	<0.5 EU/mg as determined by the LAL method
Sterility	0.2μm filtered
Storage	Stable for twelve months from date of receipt when stored at -20°C to -80°C; Stored at 2-8°C for one month without detectable loss of activity.

Characterization

Application

References

Liu Y, Gao Y, Hao H, Hou T. CD279 mediates the homeostasis and survival of regulatory T cells by enhancing T cell and macrophage interactions. FEBS Open Bio. 2020 Jun;10(6):1162-1170.
Lai CY, Tseng PC, Chen CL, Satria RD, Wang YT, Lin CF. Different Induction of PD-L1 (CD274) and PD-1 (CD279) Expression in THP-1-Differentiated Types 1 and 2 Macrophages. J Inflamm Res. 2021 Oct 13;14:5241-5249.