KB-1571

3D5-hIgG1

45940

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Background of 3D5-hIgG1

His-tag, also known as polyhistidine tag, consists of 6 to 10 consecutive histidine residues. The most commonly used his-tag is the 6xHis tag, which has a molecular weight of 0.8 kDa. This tag is used in many recombinant proteins and can aid in protein purification. The recombinant anti-His scFv is a convenient detection tool when fused to alkaline phosphatase. When immobilized on a matrix, the antibody can be used for affinity purification of recombinant proteins carrying a very short tag of just three histidine residues, suitable for crystallization. The experimental structure is now the basis for the design of antibodies with even higher stability and affinity.

Specifications

Catalog Number	KB-1571
Antibody Name	3D5-hIgG1
Isotype	Human IgG1,kappa
FC Muations	Wild Type
Target	His-Tag
Species Reactivity	Human
Host Cell Line	EXPI CHO
Purification Method	Affinity purified
Concentration	>2 mg/mL
Formulation	50 mM sodium citrate,150mM NaCl,pH5.5
Purity	>95% by SDS-PAGE and SEC-HPLC
Validation	ELISA
Endotoxin Level	<0.2 EU/mg as determined by the LAL method
Sterility	0.2μm filtered
Storage	It is recommended that the protein should be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Stability	Stable for twelve months from date of receipt when stored at -20°C to -80°C; Stored at 2-8°C for one month without detectable loss of activity.

Characterization

Application

References

Kaufmann M, Lindner P, Honegger A, Blank K, Tschopp M, Capitani G, Plückthun A, Grütter MG. Crystal structure of the anti-His tag antibody 3D5 single-chain fragment complexed to its antigen. J Mol Biol. 2002 Apr 19;318(1):135-47.