KB-1444

8C11-mIgG2a

45702

Background of 8C11-mIgG2a

Mouse Tumor Necrosis Factor-alpha (TNF-a) is a pleiotropic cytokine that plays a critical role in immune system regulation, inflammation, and cell proliferation. It is mainly produced by macrophages, T cells, and neutrophils. TNF-a acts as a chemoattractant for neutrophils, promoting their infiltration and activation at sites of inflammation. It is involved in various disease processes, including several types of cancer, where it can promote angiogenesis, cell proliferation, and inhibit apoptosis. In the central nervous system (CNS), TNF-a regulates homeostatic functions such as neurogenesis, myelination, blood-brain barrier permeability, and synaptic plasticity. However, it can also potentiate neuronal excitotoxicity and CNS inflammation, highlighting its dual role in both physiological and pathological processes.

Specifications

Catalog Number	KB-1444
Antibody Name	8C11-mIgG2a
Isotype	Mouse IgG2a,kappa
FC Muations	Wild Type
Target	Mouse TNFa
Species Reactivity	Mouse
Host Cell Line	EXPI CHO
Purification Method	Affinity purified
Concentration	>2 mg/mL
Formulation	50 mM sodium citrate,150mM NaCl,pH5.5
Purity	>95% by SDS-PAGE and SEC-HPLC
Validation	ELISA\|KD
Endotoxin Level	<0.2 EU/mg as determined by the LAL method
Sterility	0.2μm filtered
Storage	It is recommended that the protein should be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Stability	Stable for twelve months from date of receipt when stored at -20°C to -80°C; Stored at 2-8°C for one month without detectable loss of activity.

Characterization

Application

References

González-Clemente JM, Mauricio D, Richart C, Broch M, Caixàs A, Megia A, Giménez-Palop O, Simón I, Martínez-Riquelme A, Giménez-Pérez G, Vendrell J. Diabetic neuropathy is associated with activation of the TNF-alpha system in subjects with type 1 diabetes mellitus. Clin Endocrinol 63: 525–529, 2005.
Horiuchi T, Mitoma H, Harashima S, Tsukamoto H, Shimoda T. Transmembrane TNF-alpha: structure, function and interaction with anti-TNF agents. Rheumatology 49: 1215–1228, 2010.