| Catalog Number | KC-3387 |
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| Cell Line Name | Ba/F3-AKT1-D323H-C296A-Cell-Line |
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| Host Cell Line | Mouse Ba/F3 cell line |
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| Description | Stable Ba/F3 clone expressing exogenous AKT1 gene bearing D323H and C296A double amino acid mutations. |
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| Quantity | Two vials of frozen cells (≥2-106/vial) |
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| Stability | Stable in culture over a minimum of 10 passages |
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| Application | Drug screening and biological assays |
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| Freezing Medium | 70% RPMI1640+20% FBS+10% DMSO |
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| Propagation Medium | RPMI1640+10%FBS |
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| Selection Marker | Puromycin |
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| Morphology | Mostly single, round (some polymorph) cells in suspension |
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| Subculture | Split saturated culture 1:10 every 3 days; seed out at about 1-3 × 10 5 cells/mL |
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| Incubation | 37 °C with 5% CO 2 |
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| Storage | Liquid nitrogen immediately upon receiving |
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| Doubling Time | Approximately 20 hours |
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| Mycoplasma Status | Negative |
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| In Vivo Validation | NA |
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