KC-2788

Ba/F3-EGFR-B2M-HLA-A0201-Cell-Line

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Home » 细胞系 » Ba/F3-EGFR-B2M-HLA-A0201-Cell-Line

Background of Ba/F3-EGFR-B2M-HLA-A0201-Cell-Line

Specifications

Catalog NumberKC-2788
Cell Line NameBa/F3-EGFR-B2M-HLA-A0201-Cell-Line
Host Cell LineMouse Ba/F3 cell line
DescriptionStable Ba/F3 EGFR clone expressing exogenous B2M-HLA-A0201 gene.
QuantityTwo vials of frozen cells (≥2-106/vial)
StabilityStable in culture over a minimum of 10 passages
ApplicationDrug screening and biological assays
Freezing Medium70% RPMI1640+20% FBS+10% DMSO
Propagation MediumRPMI1640+10%FBS+1000µg/mL Hygromycin B
Selection MarkerHygromycin B
MorphologyMostly single, round (some polymorph) cells in suspension
SubcultureSplit saturated culture 1:10 every 3 days; seed out at about 1-3 × 105 cells/mL
Incubation37 °C with 5% CO2
StorageLiquid nitrogen immediately upon receiving
Doubling TimeApproximately 20 hours
Mycoplasma StatusNegative
In Vivo ValidationNA

Cell Line Generation

Characterization

Cell Resuscitation

Cell Freezing

References

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