Ba/F3-EPOR-JAK2-V617F-mJAK2-KO-GFP-Luc2 Cell Line

Janus kinase 2 (JAK2) belongs to the non-receptor tyrosine kinase family. JAK2 is a kinase that is misregulated or mutated in a number of myeloproliferative diseases and cancers. The mutation V617F is the most clinically relevant variant, and is seen in around half of myeloproliferative disorders. The variant is a known activating mutation, and activated JAK2 is sufficient to drive myeloproliferative disorders in mouse models. JAK2 is widely distributed in the cytoplasm of a variety of somatic cells, involved in cell-cycle regulation, apoptosis, mitotic chromosome recombination, genetic instability, and heterochromatin changes, and other biological processes. Selective knockdown of JAK2 in mice can result in embryonic anemia and death at approximately 12.5 days. JAK2 exists in the nucleus of hematopoietic cells, which can activate the transcription of H3Y41 through phosphorylation of the histone protein. The shuttle between the cytoplasm and the nucleus of JAK2 is mainly regulated by ubiquitination of JAK2. Green fluorescence protein(GFP) is a protein composed with 238 amino acids and isolated from Aequorea victoria. GFP emits green fluorescence spontaneously. Luciferase is an oxidative enzyme that can produce bioluminescence with the addition of luciferin, but don’t need an external light source, which is different from fluorescent proteins. The bioluminescence can be detected directly by light sensitive device, such as luminometer or modified microscope. Luciferase is widely used in many fields of biological research, such as transcriptional activity, kinase or other enzyme activity, cellular ATP level, whole animal imaging.