KC-3014

Ba/F3-KIF5B-RET-G810R-Luc2-Cell-Line

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Home » 细胞系 » Ba/F3-KIF5B-RET-G810R-Luc2-Cell-Line

Background of Ba/F3-KIF5B-RET-G810R-Luc2-Cell-Line

Specifications

Catalog NumberKC-3014
Cell Line NameBa/F3-KIF5B-RET-G810R-Luc2-Cell-Line
Host Cell LineMouse Ba/F3 cell line
DescriptionStable Ba/F3 clone expressing exogenous KIF5B-RET fusion protein bearing G810R mutation and luciferase.
QuantityTwo vials of frozen cells (≥2-106/vial)
StabilityStable in culture over a minimum of 10 passages
ApplicationDrug screening and biological assays
Freezing Medium70% RPMI1640+20% FBS+10% DMSO
Propagation MediumRPMI1640+10%FBS+1mg/mL hygromycin
Selection MarkerPuromycin, Hygromycin B
MorphologyMostly single, round (some polymorph) cells in suspension
SubcultureSplit saturated culture 1:10 every 3 days; seed out at about 1-3 × 105 cells/mL
Incubation37 °C with 5% CO2
StorageLiquid nitrogen immediately upon receiving
Doubling TimeApproximately 20 hours
Mycoplasma StatusNegative
In Vivo ValidationNA

Cell Line Generation

Characterization

Cell Resuscitation

Cell Freezing

References

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