KC-3014

Ba/F3-KIF5B-RET-G810R-Luc2-Cell-Line

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Home » 细胞系 » Ba/F3-KIF5B-RET-G810R-Luc2-Cell-Line

Background of Ba/F3-KIF5B-RET-G810R-Luc2-Cell-Line

Specifications

Catalog NumberKC-3014
Cell Line NameBa/F3-KIF5B-RET-G810R-Luc2-Cell-Line
Host Cell LineMouse Ba/F3 cell line
DescriptionStable Ba/F3 clone expressing exogenous KIF5B-RET fusion protein bearing G810R mutation and luciferase.
QuantityOne vial of frozen cells (≥2-106/vial)
StabilityStable in culture over a minimum of 10 passages
ApplicationDrug screening and biological assays
Freezing Medium70% RPMI1640+20% FBS+10% DMSO
Propagation MediumRPMI1640+10%FBS+1mg/mL hygromycin
Selection MarkerPuromycin, Hygromycin B
MorphologyMostly single, round (some polymorph) cells in suspension
SubcultureSplit saturated culture 1:10 every 3 days; seed out at about 1-3 × 105 cells/mL
Incubation37 °C with 5% CO2
StorageLiquid nitrogen immediately upon receiving
Doubling TimeApproximately 20 hours
Mycoplasma StatusNegative
In Vivo ValidationNA

Cell Line Generation

Characterization

Cell Resuscitation

Cell Freezing

References

Use License Agreement

Research Use Only.
Not for use in diagnostic procedures or therapeutic applications.
Redistribution of the cell line or its derivatives is prohibited without prior written permission from Kyinno Biotechnology.
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