KC-2306

CHOK1-CD93-Cell-Line

23798

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Background of CHOK1-CD93-Cell-Line

CD93 (Cluster of Differentiation 93) is a protein that in humans is encoded by the CD93 gene. CD93—a C-type lectin transmembrane protein primarily expressed in endothelial cells (ECs), immature B cells, and monocytes—has been widely reported to play important roles in vascular angiogenesis. targeting the CD93 pathway may be a safe and durable approach to improve tumor vascular functions.

Specifications

Catalog Number	KC-2306
Cell Line Name	CHOK1-CD93-Cell-Line
Host Cell Line	Chinese hamster ovary CHO-K1 cell line
Description	Stable CHO-K1 cell line expressing exogenous CD93 gene
Quantity	Two vials of frozen cells (≥2-10⁶/vial)
Stability	Stable in culture over a minimum of 10 passages
Application	Drug screening and biological assays
Freezing Medium	70% RPMI1640+20% FBS+10% DMSO
Propagation Medium	RPMI1640+10%FBS+10μg/mL Puromycin
Selection Marker	Puromycin
Morphology	Epithelial-like
Subculture	Split saturated culture 1:4-1:8 every 2-3 days; seed out at about 1-2 × 10⁵ cells/mL
Incubation	37 °C with 5% CO₂
Storage	Liquid nitrogen immediately upon receiving
Doubling Time	Approximately 24 hours
Mycoplasma Status	Negative
In Vivo Validation	NA

Cell Line Generation

CHOK1 CD93 cell line was generated using a lentiviral vector expressing human CD93 sequence.

Characterization

Cell Resuscitation

Prewarm culture medium (RPMI1640 + 10% FBS + 10μg/mL Puromycin) in a 37°C water bath.
Thaw the frozen vial in a 37°C water bath for 1-2 minutes.
Transfer the vial into biosafety cabinet, and wipe the surface with 70% ethanol.
Unscrew the top of the vial and transfer the cell suspension gently into a sterile centrifuge tube containing 9.0mL complete culture medium.
Spin at ~ 125 × g for 5-7 minutes at room temperature, and discard the supernatant without disturbing the pellet.
Resuspend cell pellet with the appropriate volume of complete medium and transfer the cell suspension into a T25 culture flask.
Incubate the flask at 37°C, 5% CO₂ incubator.
Split saturated culture 1:4-1:8 every 2-3 days; seed out at about 1-2 × 10⁵ cells/mL.

Cell Freezing

Prepare the freezing medium (70% RPMI-1640 + 20% FBS + 10% DMSO) fresh immediately before use.
Keep the freezing medium on ice and label cryovials.
Transfer cells to a sterile, conical centrifuge tube, and count the cells.
Centrifuge the cells at 250×g for 5 minutes at room temperature and carefully aspirate off the medium.
Resuspend the cells at a density of at least 3×10⁶ cells/mL in chilled freezing medium.
Aliquot 1 mL of the cell suspension into each cryovial.
Freeze cells in the CoolCell freezing container overnight in a -80°C freezer.
Transfer vials to liquid nitrogen for long-term storage

References

Nepomuceno RR, Henschen-Edman AH, Burgess WH, Tenner AJ (February 1997). "cDNA cloning and primary structure analysis of C1qR(P), the human C1q/MBL/SPA receptor that mediates enhanced phagocytosis in vitro". Immunity. 6 (2): 119Ã¿29. doi:10.1016/S1074-7613(00)80419-7. PMID 9047234.
Webster SD, Park M, Fonseca MI, Tenner AJ (January 2000). "Structural and functional evidence for microglial expression of C1qR(P), the C1q receptor that enhances phagocytosis". Journal of Leukocyte Biology. 67 (1): 109Ã¿ 16. doi:10.1002/jlb.67.1.109. PMID 10648005. S2CID 14982216
Yi Sun, Wei Chen, Lieping Chen, Yuwen Zhu (July 2021).¡ì Blockade of the CD93 pathway normalizes tumor vasculature to facilitate drug delivery and immunotherapy¡ì. SCIENCE TRANSLATIONAL MEDICINE Vol 13, Issue 604.