KC-4208

COLO205-PBRM1-KO-1A2 Cell Line

42794

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Background of COLO205-PBRM1-KO-1A2 Cell Line

The PBRM1 gene, which stands for Polybromo 1, encodes a subunit of the SWI/SNF (Switch/Sucrose Non-Fermentable) chromatin remodeling complex. This complex plays a critical role in maintaining normal chromatin structure and regulating gene expression. The encoded protein contains a bromodomain, which allows it to bind acetylated histones, and a bromo-adjacent homology (BAH) domain, which is involved in DNA binding and chromatin targeting.Mutations in PBRM1 are frequently observed in several types of cancer, most notably in clear cell renal cell carcinoma (ccRCC), where it is one of the most commonly mutated genes. Loss-of-function mutations in PBRM1 have been associated with impaired chromatin remodeling, leading to aberrant gene expression and subsequent tumorigenesis. Furthermore, PBRM1 mutations have been correlated with better prognosis in ccRCC patients, indicating its potential as a predictive biomarker.

Specifications

Catalog Number	KC-4208
Cell Line Name	COLO205-PBRM1-KO-1A2 Cell Line
Host Cell Line	COLO205
Description	Stable COLO205 clone with human PBRM1 gene knockout, No.1A2
Quantity	Two vials of frozen cells (≥2-10⁶/vial)
Stability	Stable in culture over a minimum of 10 passages
Application	Drug screening and biological assays
Freezing Medium	RPMI1640+20% FBS+10% DMSO
Propagation Medium	RPMI1640+10% FBS
Selection Marker	NA
Morphology	epithelial
Subculture	Split saturated culture 1:5-1:8 every 2-3 days; seed out at about 1-3 × 10⁵ cells/mL
Incubation	37 °C with 5% CO₂
Storage	Liquid nitrogen immediately upon receiving
Doubling Time	Approximately 27 hours
Mycoplasma Status	Negative
In Vivo Validation	NA

Cell Line Generation

Characterization

Figure 1: Characterization of COLO205-PBRM1-KO-1A2 cell line stable clone using PCR sequencing.

Figure 2: Characterization of COLO205-PBRM1-KO-1A2 cell line stable clone using RT-PCR sequencing.

Figure 3: Characterization of COLO205-PBRM1-KO-1A2 cell line stable clone using western blot.

Cell Resuscitation

Cell Freezing

References

1. Vogelstein, B., Papadopoulos, N., Velculescu, V. E., Zhou, S., Diaz, L. A., Jr., & Kinzler, K. W. (2013). Cancer genome landscapes.Science, 339(6127), 1546-1558. https://doi.org/10.1126/science.1235122
2. Vogelstein, B., Papadopoulos, N., Velculescu, V. E., Zhou, S., Diaz, L. A., Jr., & Kinzler, K. W. (2013). Cancer genome landscapes.Science, 339(6127), 1546-1558. https://doi.org/10.1126/science.1235122
3. Wang, Z., et al. (2016). PBRM1 mutations are associated with favorable prognosis and distinct immune microenvironment in clear cell renal cell carcinoma.Clinical Cancer Research, 22(12), 2889-2900. https://doi.org/10.1158/1078-0432.CCR-15-2765