KC-4210

COLO205-SETD2-KO-1D2 Cell Line

42796

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Background of COLO205-SETD2-KO-1D2 Cell Line

The SETD2 gene, encoding the SET domain containing 2 protein, is a key player in the epigenetic regulation of gene expression. This enzyme specifically methylates lysine 36 on histone H3 (H3K36me3), a modification that is critical for the proper transcriptional elongation and termination of genes, as well as for the maintenance of genomic stability. Mutations or dysregulation of SETD2 have been linked to various diseases, most notably cancers, where alterations in H3K36 methylation patterns can lead to oncogene activation and tumor suppressor gene silencing.Research has also highlighted the role of SETD2 in DNA damage response pathways and RNA processing, underscoring its multifaceted impact on cellular homeostasis. In renal cell carcinoma and other malignancies, loss-of-function mutations in SETD2 correlate with poor prognosis, suggesting its potential as a biomarker and therapeutic target.

Specifications

Catalog Number	KC-4210
Cell Line Name	COLO205-SETD2-KO-1D2 Cell Line
Host Cell Line	COLO205
Description	Stable COLO205 clone with human SETD2 gene knockout, No.1D2
Quantity	Two vials of frozen cells (≥2-10⁶/vial)
Stability	Stable in culture over a minimum of 10 passages
Application	Drug screening and biological assays
Freezing Medium	RPMI1640+20% FBS+10% DMSO
Propagation Medium	RPMI1640+10% FBS
Selection Marker	NA
Morphology	epithelial
Subculture	Split saturated culture 1:5-1:8 every 2-3 days; seed out at about 1-3 × 10⁵ cells/mL
Incubation	37 °C with 5% CO₂
Storage	Liquid nitrogen immediately upon receiving
Doubling Time	Approximately 27 hours
Mycoplasma Status	Negative
In Vivo Validation	NA

Cell Line Generation

Characterization

Figure 1: Characterization of COLO205-SETD2-KO-1D2 cell line stable clone using PCR sequencing.

Figure 2: Characterization of COLO205-SETD2-KO-1D2 cell line stable clone using RT-PCR sequencing.

Figure 3: Characterization of COLO205-SETD2-KO-1D2 cell line stable clone using western blot.

Cell Resuscitation

Cell Freezing

References

1.Fang, J., & Zhang, Y. (2014). Histone H3 lysine 36 methylation: an emerging regulator of gene expression and genome stability.Nature Reviews Molecular Cell Biology, 15(6), 371-383. https://doi.org/10.1038/nrm3803.
2.Couture, J. B., & Shilatifard, A. (2016). Histone H3K36 methylation: a double-edged sword for cancer.Nature Reviews Cancer, 16(12), 788-802. https://doi.org/10.1038/nrc.2016.109.
3.Fiorino, E., et al. (2019). SETD2 and H3K36me3: Implications for cancer and beyond.Journal of Cellular Physiology, 234(6), 7841-7852. https://doi.org/10.1002/jcp.27156