| Catalog Number | KC-6223 |
|---|---|
| Cell Line Name | HEK293-cAMP-biosensor-TSHR-Mc4-Cell-Line |
| Clone Number | 2A1 |
| Host Cell Line | HEK293-cAMP-biosensor |
| Description | Stable HEK293-cAMP-biosensor cell line expressing exogenous luciferase gene under the control of TSHR signaling pathway. |
| Quantity | One vial of frozen cells (≥2-106/vial) |
| Stability | Stable in culture over a minimum of 10 passages |
| Application | Drug screening and biological assays |
| Freezing Medium | 70% DMEM+20% FBS+10% DMSO |
| Propagation Medium | DMEM+10% FBS+150µg/mL Hygromycin B+1µg/mL Puromycin |
| Selection Marker | Puromycin | Hygromycin B |
| Morphology | Epithelial |
| Subculture | Split saturated culture 1:4-1:5 every 2-3 days; seed out at about 1-3 × 105 cells/mL |
| Incubation | 37 °C with 5% CO2 |
| Storage | Liquid nitrogen immediately upon receiving |
| Doubling Time | Approximately 30 hours |
| Mycoplasma Status | Negative |
Figure 1. HEK293-cAMP-biosensor-TSHR-Mc4 cells were seeded in 96-well plates and treated with TSH (10 µg/mL max; 3.16-fold dilutions). After adding GloSensor cAMP Reagent, cAMP signals were continuously recorded for 30 min.
Figure 2. HEK293-cAMP-biosensor-TSHR-Mc4 cells were seeded in 96-well plates and treated with M22-hIgG1 (20 µg/mL max; 3.16-fold dilutions). After adding GloSensor cAMP Reagent, cAMP signals were continuously recorded for 30 min.
Figure 3. HEK293-cAMP-biosensor-TSHR-Mc4 cells were seeded in 96-well plates, preincubated with K170-hIgG1 (20 µg/mL max; 3.16-fold dilutions) for 1 h, then stimulated with TSH (1000 ng/mL). After adding GloSensor cAMP Reagent, cAMP signals were continuously recorded for 30 min.
Figure 4. HEK293-cAMP-biosensor-TSHR-Mc4 cells were seeded in 96-well plates, preincubated with K170-hIgG1 (20 µg/mL max; 3.16-fold dilutions) for 1 h, then stimulated with M22-hIgG1 (1 µg/mL). After adding GloSensor cAMP Reagent, cAMP signals were continuously recorded for 30 min.
Figure 5. HEK293-cAMP-biosensor-TSHR-Mc4 cells were seeded in 96-well plates, preincubated with IgG1 (20 µg/mL max; 3.16-fold dilutions) for 1 h, then stimulated with TSH (1000 ng/mL). After adding GloSensor cAMP Reagent, cAMP signals were continuously recorded for 30 min.
Figure 6. HEK293-cAMP-biosensor-TSHR-Mc4 cells were seeded in 96-well plates, preincubated with IgG1 (20 µg/mL max; 3.16-fold dilutions) for 1 h, then stimulated with M22-hIgG1 (1 µg/mL). After adding GloSensor cAMP Reagent, cAMP signals were continuously recorded for 30 min.
