KB-1126

JNJ372-Met-hIgG1

12135

Home » JNJ372-Met-hIgG1

Background of JNJ372-Met-hIgG1

JNJ372-Met-hlgG1, a high affinity-specific humanized IgG1 antibody against HGFR. HGFR also known as the only high affinity receptor for hepatocyte growth factor,plays vital physiological functions during embryogenesis, angiogenesis, and repair processes.Under malignant conditions, the interaction between HGF and cMET triggers an intricate system of downstream mitogen-activated protein kinase (MAPK)/ERK and PI3K/AKT signaling cascades, thus resulting in uncontrolled cell proliferation, epithelial‐ mesenchymal transition (EMT) and metastatic spread. Antibodies to HGFR are used in humans as targeted therapies for a variety of cancers that included patients with bladder cancer,hepatocellular cancer and nonsmall cell lung cancer.

Specifications

Catalog Number	KB-1126
Antibody Name	JNJ372-Met-hIgG1
Isotype	Human IgG1,kappa
FC Muations	Wild Type
Target	MET
Species Reactivity	Human
Host Cell Line	EXPI CHO
Purification Method	Affinity purified
Concentration	>2 mg/mL
Formulation	50 mM sodium citrate,150mM NaCl,pH5.5
Purity	>95% by SDS-PAGE and SEC-HPLC
Validation	ELISA\|FACS\|KD
Endotoxin Level	<0.2 EU/mg as determined by the LAL method
Sterility	0.2μm filtered
Storage	It is recommended that the protein should be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Stability	Stable for twelve months from date of receipt when stored at -20°C to -80°C; Stored at 2-8°C for one month without detectable loss of activity.

Characterization

Application

References

Wang Siyun,Ma Haiqing,Yan Yan et al. cMET promotes metastasis and epithelial-mesenchymal transition in colorectal carcinoma by repressing RKIP.[J] J Cell Physiol, 2021, 236: 3963-3978.
Maroun,C.R.,& Rowlands,T. The Met receptor tyrosine kinase: A key player in oncogenesis and drug resistance.[J] Pharmacology and Therapeutics,2014,142(3), 316-338.