Stable NIH3T3 cell line expressing exogenous FGFR3 gene bearing S249C and V555M mutations.
| Catalog Number | KC-2842 |
|---|---|
| Cell Line Name | Jurkat-IL2P-Luc2 Cell Line |
| Host Cell Line | Jurkat |
| Description | Stable Jurkat cell line expressing exogenous luciferase gene under the control of IL2 promotor. |
| Quantity | One vial of frozen cells (≥2-106/vial) |
| Stability | Stable in culture over a minimum of 10 passages |
| Application | Cell model for monitoring IL2 signaling pathway. |
| Freezing Medium | 70% RPMI1640+20% FBS+10% DMSO |
| Propagation Medium | RPMI1640+10%FBS +300ug/ml Hygromycin B |
| Selection Marker | Hygromycin B |
| Morphology | lymphoblast |
| Subculture | Split saturated culture 1:4-1:5 every 2-3 days; seed out at about 1-3 × 105 cells/mL |
| Incubation | 37 °C with 5% CO2 |
| Storage | Liquid nitrogen immediately upon receiving |
| Doubling Time | Approximately 48 hours |
| Mycoplasma Status | Negative |
| In Vivo Validation | NA |
Figure 1. Jurkat-IL2P-Luc2 cell line was seed into the 96-well plate and treated with PMA at a maximum concentration of 30nM, and Ionomycin at a full concentration 100ng/ml diluted 3.16-fold for 16 hours, then readout with Bright-Glo system.
Figure 2. Jurkat-IL2P-Luc2 cell line was co-cultured with Raji OS8 in the 96-well plate for 16 hours, then readout with Bright-Glo system.
