| Catalog Number | KC-1902 |
|---|---|
| Cell Line Name | 293T-SMAD-Luc2 Cell Line |
| Host Cell Line | 293T |
| Description | Stable 293T cell line expressing exogenous luciferase gene under the control of SMAD transcription factors |
| Quantity | Two vials of frozen cells (≥2-106/vial) |
| Stability | Stable in culture over a minimum of 10 passages |
| Application | Drug screening and biological assays |
| Freezing Medium | 70% DMEM+20% FBS+10% DMSO |
| Propagation Medium | DMEM+10% FBS +150μg/ml Hygromycin B |
| Selection Marker | Hygromycin B |
| Morphology | Epithelial |
| Subculture | Split saturated culture 1:4-1:8 every 2-3 days; seed out at about 1-3 × 105 cells/mL |
| Incubation | 37 °C with 5% CO2 |
| Storage | Liquid nitrogen immediately upon receiving |
| Doubling Time | Approximately 30 hours |
| Mycoplasma Status | Negative |
| In Vivo Validation | NA |
Figure 1. 293T-SMAD-Luc2 cell line was seeded into the 96-well plate, and treated with TGFβ1 at a maximum concentration of 10ng/mL for 6 hours, then readout with Bright-Glo system.
Figure 2. 293T-SMAD-Luc2 cell line was seeded into the 96-well plate, and treated with TGFβ1 and M7824 at a maximum concentration of 10ng/mL and 20μg/mL for 6 hours, then readout with Bright-Glo system.
Figure 3. 293T-SMAD-Luc2 cell line was seeded into the 96-well plate, and treated with Activin A at a maximum concentration of 10μg/mL for 6 hours, then readout with Bright-Glo system.
Figure 4. 293T-SMAD-Luc2 cell line was seeded into the 96-well plate, and treated with Activin A and Bimagrumab at a maximum concentration of 50ng/mL and 20μg/mL for 6 hours, then readout with Bright-Glo system.
Figure 5. 293T-SMAD-Luc2 cells were seeded into 96-well plates, treated with Activin A for 16 hours, and then read out using Bright-Glo Detection System.
Figure 6. 293T-SMAD-Luc2 cells were seeded into 96-well plates, treated with Bimagrumab (Cat# KB-1390, Kyinno) for 1 hours, and then treated with 50ng/mL Activin A for 16 hours, and then read out using Bright-Glo Detection System.
