KC-2183

MC38-FGFR2B-Middle-Cell-Line

23550

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Background of MC38-FGFR2B-Middle-Cell-Line

FGFR2B is an epithelial-specific isoform of fibroblast growth factor receptor 2, generated by alternative splicing of the FGFR2 gene. It primarily binds to mesenchymal-derived FGF ligands (e.g., FGF7, FGF10) and plays critical roles in embryonic development, tissue repair, and epithelial homeostasis. In oncology, aberrant activation of FGFR2B—through overexpression, amplification, or ligand-dependent signaling—drives tumor progression in multiple cancers, particularly gastric, breast, and endometrial carcinomas. Its restricted expression pattern and ligand specificity make it a compelling therapeutic target, with inhibitors currently developed to block hyperactivated FGFR2B pathways in precision medicine.

Specifications

Catalog Number	KC-2183
Cell Line Name	MC38-FGFR2B-Middle-Cell-Line
Clone Number	5#
Host Cell Line	MC38
Description	Stable MC38 cell line expressing exogenous FGFR2B gene
Quantity	One vial of frozen cells (≥2-10⁶/vial)
Stability	Stable in culture over a minimum of 10 passages
Application	Drug screening and biological assays
Freezing Medium	70% DMEM+20% FBS+10% DMSO
Propagation Medium	DMEM+10% FBS +5μg/mL Puromycin
Selection Marker	Puromycin
Morphology	Epithelial
Subculture	Split saturated culture 1:4-1:8 every 2-3 days; seed out at about 1-3 × 10⁵ cells/mL
Incubation	37 °C with 5% CO₂
Storage	Liquid nitrogen immediately upon receiving
Doubling Time	Approximately 30 hours
Mycoplasma Status	Negative
In Vivo Validation	NA

Cell Line Generation

MC38-FGFR2B-Middle cell line was generated using lentivirus expressing FGFR2B sequence.

Characterization

Figure 1. Characterization of FGFR2B over-expression in the MC38-FGFR2B-Middle stable clone using FACS.[Primary antibody: Bemarituzumab(FGFR2b), Cat#KA-1202, Kyinno]

Cell Resuscitation

Prewarm culture medium (DMEM supplemented with 10% FBS, 5μg/mL Puromycin)in a 37°C water bath.
Thaw the frozen vial in a 37°C water bath for 1-2 minutes.
Transfer the vial into biosafety cabinet, and wipe the surface with 70% ethanol.
Unscrew the top of the vial and transfer the cell suspension gently into a sterile centrifuge tube containing 9.0mL complete culture medium.
Spin at ~ 125 × g for 5-7 minutes at room temperature, and discard the supernatant without disturbing the pellet.
Resuspend cell pellet with the appropriate volume of complete medium and transfer the cell suspension into a T25 culture flask.
Incubate the flask at 37°C, 5% CO₂ incubator.
Split saturated culture 1:4-1:8 every 2-3 days; seed out at about 1-3 × 10⁵ cells/mL.

Cell Freezing

Prepare the freezing medium (70% DMEM + 20% FBS + 10% DMSO) fresh immediately before use.
Keep the freezing medium on ice and label cryovials.
Transfer cells to a sterile, conical centrifuge tube, and count the cells.
Centrifuge the cells at 250×g for 5 minutes at room temperature and carefully aspirate off the medium.
Resuspend the cells at a density of at least 3×10⁶ cells/mL in chilled freezing medium.
Aliquot 1 mL of the cell suspension into each cryovial.
Freeze cells in the CoolCell freezing container overnight in a -80°C freezer.
Transfer vials to liquid nitrogen for long-term storage.

References

Tartaglia M, Valeri S, Velardi F, Di Rocco C, Battaglia PA. Trp290Cys mutation in exon IIIa of the fibroblast growth factor receptor 2 (FGFR2) gene is associated with Pfeiffer syndrome. Hum Genet. 1997 May;99(5):602-6. doi: 10.1007/s004390050413. PMID: 9150725.

Use License Agreement

Research Use Only.

Not for use in diagnostic procedures or therapeutic applications.

Redistribution of the cell line or its derivatives is prohibited without prior written permission from Kyinno Biotechnology.