KC-2983

PC3-PSMA-Cell-Line

26365

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Background of PC3-PSMA-Cell-Line

PSMA, also known as FOLH1, is a type II transmembrane glycoprotein belonging to the M28 peptidase family, which is a glutamate-preferring carboxy-peptidase. PSMA is highly expressed in the prostate secretory-acinar epithelium, in some benign extraprostatic epithelial cells from breast, duodenum, and kidney tissues, and prostate cancer. In the prostate, the protein is up-regulated in cancerous cells and is used as an effective diagnostic and prognostic indicator of prostate cancer.

Specifications

Catalog Number	KC-2983
Cell Line Name	PC3-PSMA-Cell-Line
NCBI/UniProt Accession Number	NM_004476.2
Clone Number	24#
Host Cell Line	PC3
Description	Stable PC3 cell line expressing exogenous human PSMA gene
Quantity	One vial of frozen cells (≥2-10⁶/vial)
Stability	Stable in culture over a minimum of 10 passages
Application	Drug screening and biological assays
Freezing Medium	Ham's F12K + 20% FBS + 10% DMSO
Propagation Medium	Ham's F12K + 10% FBS + 1μg/ml Puromycin
Selection Marker	Puromycin
Morphology	Epithelial
Subculture	Split saturated culture 1:4-1:8 every 2-3 days; seed out at about 1-2 × 10⁵ cells/ml
Incubation	37 °C with 5% CO₂
Storage	Liquid nitrogen immediately upon receiving
Doubling Time	Approximately 30 hours
Mycoplasma Status	Negative
In Vivo Validation	Yes

Cell Line Generation

PC3 human PSMA cell line was generated using a lentiviral vector expressing the human PSMA sequence.

Characterization

Figure1: Characterization of human PSMA overexpression in PC3 human PSMA stable clones using FACS.

Figure2: Characterization of human PSMA expression in PC3 cells using FACS.

Figure3: Characterization of PC3 human PSMA stable clone using PCR sequencing.

Cell Resuscitation

Prewarm culture medium (Ham's F12K supplemented with 10% FBS and 1μg/mL Puromycin)in a 37°C water bath.
Thaw the frozen vial in a 37°C water bath for 1-2 minutes.
Transfer the vial into biosafety cabinet, and wipe the surface with 70% ethanol.
Unscrew the top of the vial and transfer the cell suspension gently into a sterile centrifuge tube containing 9.0mL complete culture medium.
Spin at ~ 125 × g for 5-7 minutes at room temperature, and discard the supernatant without disturbing the pellet.
Resuspend cell pellet with the appropriate volume of complete medium and transfer the cell suspension into a T25 culture flask.
Incubate the flask at 37°C, 5% CO₂ incubator.
Split saturated culture 1:4-1:8 every 2-3 days; seed out at about 1-2 × 10⁵ cells/mL.

Cell Freezing

Prepare the freezing medium (Ham's F12K + 20% FBS + 10% DMSO) fresh immediately before use.
Keep the freezing medium on ice and label cryovials.
Transfer cells to a sterile, conical centrifuge tube, and count the cells.
Centrifuge the cells at 250×g for 5 minutes at room temperature and carefully aspirate off the medium.
Resuspend the cells at a density of at least 3×10⁶ cells/mL in chilled freezing medium.
Aliquot 1 mL of the cell suspension into each cryovial.
Freeze cells in the CoolCell freezing container overnight in a -80°C freezer.
Transfer vials to liquid nitrogen for long-term storage

References

Prostate-Specific Membrane Antigen (PSMA) Theranostics for Treatment of Oligometastatic Prostate Cancer. PMID: 34829977 PMCID: PMC8621856 DOI: 10.3390/ijms222212095.

Use License Agreement

Research Use Only.

Not for use in diagnostic procedures or therapeutic applications.

Redistribution of the cell line or its derivatives is prohibited without prior written permission from Kyinno Biotechnology.