| Catalog Number | KC-4317 |
|---|---|
| Cell Line Name | TF-1-NFκB-Luc2-DR3-Cell-Line |
| Host Cell Line | TF-1-NFκB-Luc2 |
| Description | Stable TF-1 cell line expressing exogenous luciferase gene under the control of DR3 signaling pathway |
| Quantity | Two vials of frozen cells (≥2-106/vial) |
| Stability | Stable in culture over a minimum of 10 passages |
| Application | Drug screening and biological assays |
| Freezing Medium | RPMI1640 + 20% FBS + 10% DMSO |
| Propagation Medium | RPMI1640 + 10% FBS+5ng/mL rhGM-CSF+100μg/mL Hygromycin B+1μg/mL puromycin |
| Selection Marker | Hygromycin B and puromycin |
| Morphology | Mostly single, round (some polymorph) cells in suspension |
| Subculture | Split saturated culture 1:4-1:8 every 2-3 days; seed out at about 1-3 × 105 cells/mL |
| Incubation | 37 °C with 5% CO2 |
| Storage | Liquid nitrogen immediately upon receiving |
| Doubling Time | Approximately 30 hours |
| Mycoplasma Status | Negative |
| In Vivo Validation | NA |
Figure 1. TF-1-NFκB-Luc2-DR3 cell line was seeded into the 96-well plate, and treated with TL1A at a maximum concentration of 1μg/mL for 6 hours, then readout with Bright-Glo system.
Figure 2. TF-1-NFκB-Luc2-DR3 cell line was seeded into the 96-well plate, and treated with PF-06480605-hIgG1 at a maximum concentration 20μg/mL for 1 hours, then treated with TL1A (100ng/mL), incubated for 6 hours and readout with Bright-Glo system.
