SP2/0-mRosa26-mCherry-luc2-KI Cell Line

Based on the previously constructed SP2/0-mRosa26-Lox71-GFP-Lox2272 tool cell line, we utilized the Cre-LoxP heterotypic recombination system to perform precise site-specific substitution of the GFP expression cassette, ultimately generating a stable mCherry-luc2 dual-labeled cell line. The successful cassette replacement fully validates the reliability and efficiency of this engineered cell platform, which supports accurate, unidirectional, and irreversible exogenous gene recombination without altering the endogenous genomic background. Benefiting from genetic modification at the mRosa26 genomic safe harbor locus, this cell editing system exhibits excellent genetic stability and consistent transgene expression, effectively avoiding the adverse effects of random integration, gene silencing, and variable expression levels caused by traditional cell engineering strategies. The established mCherry-luc2 dual-reporter cell line integrates fluorescent visual labeling and bioluminescent quantitative tracing functions, endowing it with versatile application potential in basic and translational research. This standardized cell model enables real-time visualization and quantitative monitoring of tumor cell proliferation, migration, and invasion both in vitro and in vivo. Furthermore, it provides a robust and reliable experimental platform for exploring the molecular mechanisms underlying myeloma progression, conducting non-invasive longitudinal tumor imaging in animal models, and performing high-throughput screening and efficacy evaluation of anti-myeloma and anti-tumor drugs. In addition, this flexible gene replacement system can be further applied to subsequent genetic modification, target gene function verification, and recombinant protein engineering research, offering a universal and standardized tool system for preclinical tumor research and drug development.