KC-6313

293T-STAT4-Luc2 Cell Line

61455

Home » 细胞系 » 293T-STAT4-Luc2 Cell Line

Background of 293T-STAT4-Luc2 Cell Line

The protein encoded by this gene is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is essential for mediating responses to IL12 in lymphocytes, and regulating the differentiation of T helper cells. Mutations in this gene may be associated with systemic lupus erythematosus and rheumatoid arthritis. Alternate splicing results in multiple transcript variants that encode the same protein. STAT4 (Signal Transducer And Activator Of Transcription 4) is a Protein Coding gene. Diseases associated with STAT4 include Disabling Pansclerotic Morphea Of Childhood and Systemic Lupus Erythematosus 11. Among its related pathways are Interleukin-12 family signaling and IL27-mediated signaling events. Gene Ontology (GO) annotations related to this gene include DNA-binding transcription factor activity and sequence-specific DNA binding.

Specifications

Catalog Number	KC-6313
Cell Line Name	293T-STAT4-Luc2 Cell Line
Clone Number	4B1
Host Cell Line	293T
Description	Stable 293T cell line expressing exogenous luciferase under the control of STAT4 responsive element.
Quantity	Two vials of frozen cells (≥2-10⁶/vial)
Stability	Stable in culture over a minimum of 10 passages
Application	Drug screening and biological assays
Freezing Medium	70% DMEM + 20% FBS + 10% DMSO
Propagation Medium	DMEM + 10% FBS + 75μg/mL Hygromycin B + 0.5μg/mL puromycin
Selection Marker	Hygromycin B and Puromycin
Morphology	Epithelial
Subculture	Split saturated culture 1:4-1:6 every 2-3 days; seed out at about 1 × 10⁵ cells/mL
Incubation	37 °C with 5% CO₂
Storage	Liquid nitrogen immediately upon receiving
Doubling Time	Approximately 30 hours
Mycoplasma Status	Negative

Cell Line Generation

293T-STAT4-Luc2 Cell Line was generated using a lentiviral vector expressing the STAT4-Luc2 sequence.

Characterization

Figure 1: 293T-STAT4-Luc2 cell were seeded into 96-well plates, treated with IFN-γ in different concentrations for 16 hours, and then read out using Bright-Glo Detection System.

Cell Resuscitation

Prewarm culture medium (DMEM supplemented with 10% FBS, 75μg/mL Hygromycin B and 0.5μg/mL Puromycin) in a 37°C water bath.
Thaw the frozen vial in a 37°C water bath for 1-2 minutes.
Transfer the vial into biosafety cabinet, and wipe the surface with 70% ethanol.
Unscrew the top of the vial and transfer the cell suspension gently into a sterile centrifuge tube containing 9.0mL complete culture medium.
Spin at ~ 125 × g for 5-7 minutes at room temperature, and discard the supernatant without disturbing the pellet.
Resuspend cell pellet with the appropriate volume of complete medium and transfer the cell suspension into a T25 culture flask.
Incubate the flask at 37°C, 5% CO₂ incubator.
Split saturated culture 1:4-1:6 every 2-3 days; seed out at about 1 × 10⁵ cells/mL.

Cell Freezing

Prepare the freezing medium (DMEM + 20% FBS + 10% DMSO) fresh immediately before use.
Keep the freezing medium on ice and label cryovials.
Transfer cells to a sterile, conical centrifuge tube, and count the cells.
Centrifuge the cells at 250×g for 5 minutes at room temperature and carefully aspirate off the medium.
Resuspend the cells at a density of at least 3×10⁶ cells/mL in chilled freezing medium.
Aliquot 1 mL of the cell suspension into each cryovial.
Freeze cells in the CoolCell freezing container overnight in a -80°C freezer.
Transfer vials to liquid nitrogen for long-term storage.

References

Baghdassarian H, Blackstone SA, Clay OS, Philips R, Matthiasardottir B, Nehrebecky M, Hua VK, McVicar R, Liu Y, Tucker SM, Randazzo D, Deuitch N, Rosenzweig S, Mark A, Sasik R, Fisch KM, Pimpale Chavan P, Eren E, Watts NR, Ma CA, Gadina M, Schwartz DM, Sanyal A, Werner G, Murdock DR, Horita N, Chowdhury S, Dimmock D, Jepsen K, Remmers EF, Goldbach-Mansky R, Gahl WA, O'Shea JJ, Milner JD, Lewis NE, Chang J, Kastner DL, Torok K, Oda H, Putnam CD, Broderick L. Variant STAT4 and Response to Ruxolitinib in an Autoinflammatory Syndrome. N Engl J Med. 2023 Jun 15;388(24):2241-2252. doi: 10.1056/NEJMoa2202318. Epub 2023 May 31. PMID: 37256972; PMCID: PMC10392571.
National Center for Biotechnology Information (2026). PubChem Pathway Summary for Pathway il12_stat4pathway, IL12 signaling mediated by STAT4, Source: Pathway Interaction Database. Retrieved February 4, 2026 from https://pubchem.ncbi.nlm.nih.gov/pathway/Pathway-Interaction-Database:il12_stat4pathway.
Zinser E, Tan KL, Kim DS, O'Brien R, Winstanley A, Yong PFK. Differential Diagnosis: Hepatic Complications in Inborn Errors of Immunity. J Clin Med. 2023 Dec 3;12(23):7480. doi: 10.3390/jcm12237480. PMID: 38068532; PMCID: PMC10707526.