KB-1530

LAE102-hIgG1

45862

Background of LAE102-hIgG1

ACVR2A is a type II member of the receptor Serine/Threonine kinases in the TGF β family. It serves as a receptor for Activin A, Activin B, and Inhibin A. Signaling by activins and BMPs is highly promiscuous. Apart from signaling through ALK4/7, the activin type II receptors (ACVR2A and ACVR2B) can also interact with several type I BMP receptors (ALK1/2/3/6), which can in turn form complexes with the type II BMP receptor, BMPRII. Type II receptors phosphorylate and activate type I receptors, which then autophosphorylate and bind and activate SMAD transcriptional regulators. ACVR2A can form complexes with different type I receptors that signal either to Smad2/3 (ALK4) or to Smad1/5/8 (ALK2, ALK3, ALK6). The different type I receptors compete for binding to ACVR2A, and this competition provides a mechanism that balances signaling between Activin A mediated, ALK4 dependent Smad2/3 signaling and BMP mediated ALK2 or ALK3 dependent signaling to Smad1/5/8. In myeloma cells, activin A treatment inhibits BMP 6 and BMP 9 induced activation of SMAD1/5/8 through ACVR2A/ACVR2B/ALK2.

Specifications

Catalog Number	KB-1530
Antibody Name	LAE102-hIgG1
Isotype	Human IgG1,kappa
FC Muations	Wild Type
Target	ACVR2A
Species Reactivity	Human
Host Cell Line	EXPI CHO
Purification Method	Affinity purified
Concentration	>2 mg/mL
Formulation	50 mM sodium citrate,150mM NaCl,pH5.5
Purity	>95% by SDS-PAGE and SEC-HPLC
Validation	ELISA
Endotoxin Level	<0.2 EU/mg as determined by the LAL method
Sterility	0.2μm filtered
Storage	It is recommended that the protein should be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Stability	Stable for twelve months from date of receipt when stored at -20°C to -80°C; Stored at 2-8°C for one month without detectable loss of activity.

Characterization

Application

References

Oddrun Elise Olsen, et al. Activin A inhibits BMP signaling by binding ACVR2A and ACVR2B. Cell Commun Signal. 2015 Jun 6;13:27.
V K Chin, et al. Inhibition of Activin A suppressed tumor necrosis factor α secretion and improved histopathological conditions in malarial mice. Trop Biomed. 2021 Mar 1;38(1):187 204. .
Szabina Szófia Szilágyi, et al. Competition between type I activin and BMP receptors for binding to ACVR2A regulates signaling to distinct Smad pathways. BMC Biol. 2022 Feb 18;20(1):50. .
Heather L Hayes, et al. A Pdx 1 Regulated Soluble Factor Activates Rat and Human Islet Cell Proliferation. Mol Cell Biol. 2016 Nov 14;36(23):2918 2930. .